Superoxide dismutase (SOD) as a free radical scavenger in Porphyromonas gingivalis is well documented. The aim of this work was to develop an enzyme-linked immunosorbent assay using recombinant P. gingivalis SOD as an antigen. The sandwich complex was detected using a secondary antibody conjugated to β-D-galactosidase. Under optimum conditions, the sensitivity and the limit of detection were determined from 25pg to 500pg. In future, the application will be extended to the expression of SOD from P. gingivalis under various growing conditions.
Immunoassay based on a polyclonal antibody for the quantification of Porphyromonas gingivalis SOD : a preliminary report
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