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Purification and Partial Characterization of Leucine Aminopeptidase from Actinomyces viscosus
https://mdu.repo.nii.ac.jp/records/1381
https://mdu.repo.nii.ac.jp/records/13815715a91c-a873-4444-b2f8-028952009d0b
名前 / ファイル | ライセンス | アクション |
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matsumoto_shigaku_10-02-03.pdf (505.6 kB)
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Item type | Journal Article(1) | |||||
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公開日 | 2012-05-24 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Purification and Partial Characterization of Leucine Aminopeptidase from Actinomyces viscosus | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Leucine aminopeptidase | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | purification | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | A.viscosus | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
NAKAMURA, TAKESHI
× NAKAMURA, TAKESHI |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Leucine aminopeptidase was purified from cell-free extracts of Actinomyces viscosus ATCC 19246 and some properties were investigated. The enzyme had a molecular weight of 65,000 and its isoelectric point was 4.0. Optimum pH was found at 7.0. The enzyme was quite labile over 40℃. It was sensitive to inhibition by diisopropylfluorophosphate, phenylmethane sulfonylfluoride, or tosyl-L-lysine chloromethyl ketone. Inhibition by urea was also obvious and this inhibition was found to be irreversible. Ca^<2+>, Mg^<2+>, Mn^<2+> or Co^<2+> had no no effect on the activity. Leucine-P-nitroanilide was the most suitable substrate among the tested synthetic substrates. | |||||
書誌情報 |
松本歯学 巻 10, 号 2, p. 130-135, 発行日 1984-12-31 |
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出版者 | ||||||
出版者 | 松本歯科大学学会 | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0385-1613 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AN00232590 | |||||
フォーマット | ||||||
内容記述タイプ | Other | |||||
内容記述 | application/pdf | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 |