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Effect of substituting Trp for Leu at position 72 on the structure of Porphyromonas gingivalis superoxide dismutase
https://mdu.repo.nii.ac.jp/records/2268
https://mdu.repo.nii.ac.jp/records/226866de3e72-4274-4d4b-8860-5a920d2aab2e
名前 / ファイル | ライセンス | アクション |
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matsumoto_shigaku40-1-03.pdf (2.9 MB)
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Item type | 松本歯学/学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2014-09-11 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Effect of substituting Trp for Leu at position 72 on the structure of Porphyromonas gingivalis superoxide dismutase | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | site–directed mutagenesis | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | superoxide dismutase | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Porphyromonas gingivalis | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
その他(別言語等)のタイトル | ||||||
その他のタイトル | Porphyromonas gingivalis スーパーオキシドジスムターゼの構造における72位Leu をTrp に置換した影響 | |||||
著者 |
KOMACHIYA, MIHO
× KOMACHIYA, MIHO× MIZOUE, SHINYA× MIHARA, MASASHI× OSAWA, MASAKI× KIKUCHI, YUICHIRO× UEMATSU, SETSUKO× UEMATSU, SETSUKO× OKUBO, YUICHIRO× KUROIWA, AKIHIRO× YAMADA, KAZUHIRO× YAMAKURA, FUMIYUKI× HIRAOKA, B.YUKIHIRO |
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著者別名 |
小町谷, 美帆
× 小町谷, 美帆× 溝上, 真也× 三原, 正志× 大澤, 雅樹× 菊池, 有一郎× 上松, 節子× 平井, 要× 大久保, 裕一郎× 黒岩, 昭弘× 山田, 一尋× 山倉, 文幸× 平岡, 行博 |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Porphyromonas gingivalis contains a single constitutive superoxide dismutase (SOD) that is active with either iron or manganese at the active site. The aim of this work was to evaluate the effect of the Leu ₇2 to Trp mutation on the structure of P. gingivalis SOD (Pg SOD) using lectrophoretic characterization. Leu ₇2, which is located near the active site metal, is substituted with Trp in aligned amino acid sequences of iron–containing SOD. The results of electrophoretic characterization and the expressed activity of mutant SOD suggest that mutant SOD have the same gross structure as wild–type SOD. We herein conclude that the integrity of Leu ₇2 is a necessary requisite for the metal–tolerant activity of Pg SOD. | |||||
書誌情報 |
松本歯学 en : Journal of the Matsumoto Dental University Society 巻 40, 号 1, p. 19-25, 発行日 2014-06-30 |
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出版者 | ||||||
出版者 | 松本歯科大学学会 | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0385-1613 | |||||
フォーマット | ||||||
内容記述タイプ | Other | |||||
内容記述 | application/pdf | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 |