| Item type |
松本歯学/学術雑誌論文 / Journal Article(1) |
| 公開日 |
2016-09-29 |
| タイトル |
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タイトル |
Proteome Analysis Identifies the Dpr Protein of Streptococcus mutans as an Important Factor in the Presence of Early Streptococcal Colonizers of Tooth Surfaces |
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言語 |
en |
| 言語 |
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|
言語 |
eng |
| キーワード |
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言語 |
en |
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主題Scheme |
Other |
|
主題 |
Bacterial Proteins |
| キーワード |
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言語 |
en |
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主題Scheme |
Other |
|
主題 |
Biofilms |
| キーワード |
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|
言語 |
en |
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主題Scheme |
Other |
|
主題 |
Electrophoresis Gel Two-Dimensional |
| キーワード |
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言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Humans |
| キーワード |
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言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Proteomics |
| キーワード |
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|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Streptococcus gordonii |
| キーワード |
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|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Streptococcus mutans |
| キーワード |
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|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Tooth |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| 著者 |
Yoshida, Akihiro
Niki, Mamiko
Yamamoto, Yuji
Yasunaga, Ai
Anzai, Toshihiro
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| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
Oral streptococci are primary colonizers of tooth surfaces and Streptococcus mutans is the principal causative agent of dental caries in humans. A number of proteins are involved in the formation of monospecies biofilms by S. mutans. This study analyzed the protein expression profiles of S. mutans biofilms formed in the presence or absence of S. gordonii, a pioneer colonizer of the tooth surface, by two-dimensional gel electrophoresis (2-DE). After identifying S. mutans proteins by Mass spectrometric analysis, their expression in the presence of S. gordonii was analyzed. S. mutans was inoculated with or without S. gordonii DL1. The two species were compartmentalized using 0.2-μl Anopore membranes. The biofilms on polystyrene plates were harvested, and the solubilized proteins were separated by 2-DE. When S. mutans biofilms were formed in the presence of S. gordonii, the peroxide resistance protein Dpr of the former showed 4.3-fold increased expression compared to biofilms that developed in the absence of the pioneer colonizer. In addition, we performed a competition assay using S. mutans antioxidant protein mutants together with S. gordonii and other initial colonizers. Growth of the dpr-knockout S. mutans mutant was significantly inhibited by S. gordonii, as well as by S. sanguinis. Furthermore, a cell viability assay revealed that the viability of the dpr-defective mutant was significantly attenuated compared to the wild-type strain when co-cultured with S. gordonii. Therefore, these results suggest that Dpr might be one of the essential proteins for S. mutans survival on teeth in the presence of early colonizing oral streptococci. |
| 書誌情報 |
en : PLoS One
巻 10,
号 3,
p. e0121176,
発行日 2015-03-27
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| 出版者 |
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出版者 |
PLOS (Public Library of Science) |
| ISSN |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
1932-6203 |
| PubMed番号 |
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関連タイプ |
isIdenticalTo |
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識別子タイプ |
PMID |
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関連識別子 |
info:pmid/25816242 |
| DOI |
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関連タイプ |
isIdenticalTo |
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識別子タイプ |
DOI |
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関連識別子 |
https://doi.org/10.1371/journal.pone.0121176 |
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関連名称 |
info:doi/10.1371/journal.pone.0121176 |
| 権利 |
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権利情報 |
Copyright: © 2015 Yoshida et al. PLoS one |
| 情報源 |
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関連名称 |
雑誌掲載論文(電子版) |
| 関連サイト |
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識別子タイプ |
URI |
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関連識別子 |
http://dx.plos.org/10.1371/journal.pone.0121176 |
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関連名称 |
PLOS |
| フォーマット |
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内容記述タイプ |
Other |
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内容記述 |
application/pdf |
| 著者版フラグ |
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出版タイプ |
VoR |
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出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
本文 (2.5 MB)
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