2026-03-07T00:30:40Z https://mdu.repo.nii.ac.jp/oai

oai:mdu.repo.nii.ac.jp:00000098 2023-06-19T08:45:47Z 1:17:18

Immunoassay based on a polyclonal antibody for the quantification of Porphyromonas gingivalis SOD : a preliminary report 一抗体免疫法によるPorphyromonas. gingivalis SODの定量(プレリミナリーレポート) OSAWA, MASAKI MIHARA, MASASHI KIKUCHI, YUICHIRO OKUBO, YUICHIRO YAMADA, KAZUHIRO HIRAI, KANAME HIRAOKA, B YUKIHIRO ELISA superoxide dismutase Porphyromonas gingivalis application/pdf Superoxide dismutase (SOD) as a free radical scavenger in Porphyromonas gingivalis is well documented. The aim of this work was to develop an enzyme-linked immunosorbent assay using recombinant P. gingivalis SOD as an antigen. The sandwich complex was detected using a secondary antibody conjugated to β-D-galactosidase. Under optimum conditions, the sensitivity and the limit of detection were determined from 25pg to 500pg. In future, the application will be extended to the expression of SOD from P. gingivalis under various growing conditions. 松本歯科大学学会 2010-04-30 eng journal article VoR https://mdu.repo.nii.ac.jp/records/98 AN00232590 0385-1613 松本歯学 36 1 1 6 https://mdu.repo.nii.ac.jp/record/98/files/matsumoto_shigaku_36-01-01.pdf application/pdf 339.7 kB 2012-05-22