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Cytological Kinetics of Periodontal Ligament in an Experimental Occlusal Trauma Model 実験的咬合性外傷による歯根膜の細胞動態 髙谷, 達夫 Takaya, Tatsuo Hiroaki, Mimura Matsuda, Saeka Nakano, Keisuke Tsujigiwa, Hidetsugu Tomida, Mihoko Okafuji, Norimasa Fujii, Takeo Kawakami, Toshiyuki open access Occlusal trauma Periodontal tissue Green fluorescent protein(GFP) Ki67 Mouse 2015 甲第174号 application/pdf Using a model of experimental occlusal trauma in mice, we investigated cytological kinetics of periodontal ligament by means of histopathological, immunohistochemical, and photographical analysis methods. At furcation areas of molar teeth in the experimental group on day 4, the increase of cells in periodontal ligament was seen. The cells with a round-shaped nucleus were deeply stained in the hematoxylin-eosin. Ki67 positive nuclei showed a prominent increase in the experimental groups on days 4 and 7. Green Fluorescent Protein (GFP) positivity also revealed cell movement, but this action was slightly slow compared to Ki67 staining specimens. It was indicated that the homeostasis is mechanism of periodontal ligament receiving the excessive occlusal loading was promoted by osteoclasts and macrophages from bone-marrow-derived cells migrated to the periodontal ligament. The remodeling of periodontal ligament with the acceleration of cell growth was evoked from the experiment for the group on day 4 and after day 7. The findings suggested that periodontal ligament at furcation areas of molar teeth in occlusal trauma model was recovered by using both of the cells in situ and the bone-marrow-derived cells. eng doctoral thesis VoR https://mdu.repo.nii.ac.jp/records/2504 http://www.medsci.org/v12p0544.htm Ivyspring International Publisher http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4502058/ PubMed Central® (PMC) 投稿雑誌：Takaya, T. et al. Cytological Kinetics of Periodontal Ligament in an Experimental Occlusal Trauma Model. International Journal of Medical Sciences. 2015; 12(7): 544-551. © 2015 Ivyspring International Publisher. Reproduction is permitted for personal, noncommercial use, provided that the article is in whole, unmodified, and properly cited. 甲第174号 博士（歯学） 2016-02-05 松本歯科大学