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The Phagocytic Cells in the Periodontal Membrane 歯根膜内の貪食能を有する細胞について 中村, 康洋 芦澤, 雄二 出口, 敏雄 長谷川, 亨 佐原, 紀行 鈴木, 和夫 貪食細胞 HRP 歯根膜 マクロファージ application/pdf To evaluate phagocytic cells in periodontal membrane under physiological condition, we used horseradish peroxidase (HRP) as a phagocytic tracer. 10-week-old male rats were injected intravenously with HRP, and sacrificed 1 hour after the injection. Upper jaws were dissected out, decalcified, and prepared for light and electron microscopic observations. Furthermore, immunohistochemical localization of macrophages in periodontal membrane was examined by using anti-rat macrophage monoclonal antibody, ED 2. The following results were obtained: 1. Most of the phagocytic cells in the periodontal membrane can be found adjacent to the blood capillaries. 2. Electron microscopic observation revealed that most of these phagocytic cells were macrophages which had microvilli, secondary lysosomes with HRP and components of lipid-like drops in cytoplasm. 3. ED2 positive cells were found adjacent to the blood vessels in the periodontal membrane. The results of this study suggest that phagocytic cells of HRP in the periodontal membrane are macrophages. journal article 松本歯科大学学会 1998-12-31 VoR application/pdf 松本歯学 3 24 262 268 AN00232590 0385-1613 https://mdu.repo.nii.ac.jp/record/720/files/matsumoto_shigaku_24-03-03.pdf https://mdu.repo.nii.ac.jp/records/720 jpn