{"created":"2023-06-19T07:38:07.283990+00:00","id":1089,"links":{},"metadata":{"_buckets":{"deposit":"b59d44bb-82fa-497f-9055-1db862d65b37"},"_deposit":{"created_by":3,"id":"1089","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"1089"},"status":"published"},"_oai":{"id":"oai:mdu.repo.nii.ac.jp:00001089","sets":["1:45:82"]},"author_link":["0"],"item_2_alternative_title_1":{"attribute_name":"その他（別言語等）のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"Purification and Characterization of Neuraminidase from Oral Propionibacterium acnes"}]},"item_2_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1990-12-31","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"3","bibliographicPageEnd":"284","bibliographicPageStart":"276","bibliographicVolumeNumber":"16","bibliographic_titles":[{"bibliographic_title":"松本歯学"}]}]},"item_2_description_19":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_2_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"We found that an oral strain of Propionibacterium acnes (EXC-1) produced neur-aminidase in the culture supernatant. It was purified from the culture medium to homogene-ity by a sequential procedure, including ammonium sulfate precipitation, ion exchange chromatography, gel filtration, and hydrophobic chromatography. The purified sample showed a single protein band on SDS-PAGE. The enzyme was purified 2,400-fold with a recovery of 27.7%. The molecular weight was estimated to be 75,000. The optimum pH for the activity was 5.0. This enzyme was heat labile ; the activity was lost by heating at 55°C for 10 min. Ca＾<2+> at lmM increased the activity about twofold, and inhibition of the activity was observed by treatment with EDTA.","subitem_description_type":"Abstract"}]},"item_2_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"松本歯科大学学会"}]},"item_2_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00232590","subitem_source_identifier_type":"NCID"}]},"item_2_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0385-1613","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"志村, 隆二"},{"creatorName":"柴田, 幸永"},{"creatorName":"藤村, 節夫"},{"creatorName":"中村, 武"}],"nameIdentifiers":[{"nameIdentifier":"0","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2012-05-24"}],"displaytype":"detail","filename":"matsumoto_shigaku_16-03-03.pdf","filesize":[{"value":"733.6 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"matsumoto_shigaku_16-03-03.pdf","url":"https://mdu.repo.nii.ac.jp/record/1089/files/matsumoto_shigaku_16-03-03.pdf"},"version_id":"49f84fa8-1131-45e5-bb72-f2a2f64e43f6"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"口腔細菌","subitem_subject_scheme":"Other"},{"subitem_subject":"口股P.acnes","subitem_subject_scheme":"Other"},{"subitem_subject":"産生酵素","subitem_subject_scheme":"Other"},{"subitem_subject":"ノイラミニダーゼ","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"口腔Propionibacterium acnesのノイラミニダーゼの精製とその性状","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"口腔Propionibacterium acnesのノイラミニダーゼの精製とその性状"}]},"item_type_id":"2","owner":"3","path":["82"],"pubdate":{"attribute_name":"公開日","attribute_value":"2012-05-24"},"publish_date":"2012-05-24","publish_status":"0","recid":"1089","relation_version_is_last":true,"title":["口腔Propionibacterium acnesのノイラミニダーゼの精製とその性状"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-06-19T08:22:33.441987+00:00"}