@article{oai:mdu.repo.nii.ac.jp:00001119,
 author = {半戸, 茂友 and 山岸, 眞弓美 and 北村, 豊},
 issue = {1},
 journal = {松本歯学},
 month = {Apr},
 note = {application/pdf, An evaluation of a reagent strip (BM Test 6L: Boehringer-Mannheim Japan) for detecting leukocytes in urine (pyuria) is described. Detection is based on the esterase activity of leukocytes. Upon contact between the reagent matrix and leukocytes in urine, the substrate (indoxyl ester) is hydrolyzed by the esterase to indoxyl, which then couples with a diazonium salt to produce a purple azo dye. A total of 418 urine specimens were analyzed with the reagent strip, and by sediment microscopic examination for comparison. Using ten or more cells per high-power field as denoting significant pyuria, sensitivity and specificity of the strip were 85.5% and 83.4%, respectively. From these results, this reagent strip is considered a useful method for detecting leukocytes in urine. According to the results of interference studies, however, esterase activity was slightly affected by urinary pH, specific gravity, protein, and ascorbic acid.},
 pages = {44--50},
 title = {エステラーゼ活性を利用した尿中白血球検出試験紙の検討},
 volume = {16},
 year = {1990}
}