@article{oai:mdu.repo.nii.ac.jp:00001150,
 author = {中村, 武 and 志村, 隆二 and 柴田, 幸永 and 藤村, 節夫},
 issue = {2},
 journal = {松本歯学},
 month = {Aug},
 note = {application/pdf, Five bacterial strains which showed intense antimicrobial activity against Bacterioides gingivalis were isolated from gingival crevice materials. These bacteria were identified as Streptococcus sanguis based on their biological properties. The antimicrobial agent was found to be cell associated. Using one of the isolates (strain NF-10),the agent purified (from a cell extract prepared by ultrasonic treatment), by means of Q-Sepharose chromatography, gel filtration on Sephacryl S-300, further chromatography on hydroxylapatite column, and preparative polyacrylamide gel electrophoresis. A single stained protein band was observed in the PAGE of the purified antimicrobial agent. The specific activity increased 83.9-fold and the recovery was 15.3%. Its molecuar weight was 74,000 and pI was 4.5. The activity was lost on heating at 60℃ for 10 min. The action spectrum of this antimicrobial agent was narrow; of the 18 bacterial species examined, only B.gingivalis and B.intermedius were found to be inhibited by it.},
 pages = {173--181},
 title = {黒色色素産生Bacteroidesに対する口腔レンサ球菌の抗菌活性,抗菌物質の精製とその性状},
 volume = {15},
 year = {1989}
}