{"created":"2023-06-19T07:38:24.931445+00:00","id":1381,"links":{},"metadata":{"_buckets":{"deposit":"d775bca1-5d69-479e-a166-8d584fa86868"},"_deposit":{"created_by":3,"id":"1381","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"1381"},"status":"published"},"_oai":{"id":"oai:mdu.repo.nii.ac.jp:00001381","sets":["1:51:66"]},"author_link":["0"],"item_2_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1984-12-31","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"135","bibliographicPageStart":"130","bibliographicVolumeNumber":"10","bibliographic_titles":[{"bibliographic_title":"松本歯学"}]}]},"item_2_description_19":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_2_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Leucine aminopeptidase was purified from cell-free extracts of Actinomyces viscosus ATCC 19246 and some properties were investigated. The enzyme had a molecular weight of 65,000 and its isoelectric point was 4.0. Optimum pH was found at 7.0. The enzyme was quite labile over 40℃. It was sensitive to inhibition by diisopropylfluorophosphate, phenylmethane sulfonylfluoride, or tosyl-L-lysine chloromethyl ketone. Inhibition by urea was also obvious and this inhibition was found to be irreversible. Ca^<2+>, Mg^<2+>, Mn^<2+> or Co^<2+> had no no effect on the activity. Leucine-P-nitroanilide was the most suitable substrate among the tested synthetic substrates.","subitem_description_type":"Abstract"}]},"item_2_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"松本歯科大学学会"}]},"item_2_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00232590","subitem_source_identifier_type":"NCID"}]},"item_2_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0385-1613","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"NAKAMURA, TAKESHI"},{"creatorName":"KIUCHI, NAOTO"},{"creatorName":"FUJIMURA, SETSUO"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2012-05-24"}],"displaytype":"detail","filename":"matsumoto_shigaku_10-02-03.pdf","filesize":[{"value":"505.6 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"matsumoto_shigaku_10-02-03.pdf","url":"https://mdu.repo.nii.ac.jp/record/1381/files/matsumoto_shigaku_10-02-03.pdf"},"version_id":"4397b099-0f9b-4568-8c8f-b6cac2c7b148"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Leucine aminopeptidase","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"purification","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"A.viscosus","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Purification and Partial Characterization of Leucine Aminopeptidase from Actinomyces viscosus","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Purification and Partial Characterization of Leucine Aminopeptidase from Actinomyces viscosus","subitem_title_language":"en"}]},"item_type_id":"2","owner":"3","path":["66"],"pubdate":{"attribute_name":"公開日","attribute_value":"2012-05-24"},"publish_date":"2012-05-24","publish_status":"0","recid":"1381","relation_version_is_last":true,"title":["Purification and Partial Characterization of Leucine Aminopeptidase from Actinomyces viscosus"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-06-19T08:15:13.600653+00:00"}