@article{oai:mdu.repo.nii.ac.jp:00001538,
 author = {河瀬, 聡一朗 and 宮沢, 裕夫 and 平井, 要 and 金子, 仁子},
 issue = {1},
 journal = {松本歯学},
 month = {Apr},
 note = {application/pdf, In children and adults with severe psychosomatic disorders accompanied by eating and swallowing disorders, saliva contains a high number of bacteria, particularly after assisted brushing, and retention of the etiologic agent of opportunistic infection increases the risk of aspiration pneumonia. Disturbance of mouth opening and body movement interferes with
assisted brushing in these people. Moreover, severe caries and periodontal disease are caused by malalignment and adverse effects of regularly used drugs in many cases. However,
it is difficult for them to complain of the condition by themselves, leading to aggravation. Studies on bacteriostatic and bactericidal actions of natural components considered to be highly safe for the human body have recently been progressing. An extract of Eucalyptus globulus, 1,8−cineol, has been reported to exhibit a fungistatic effect, in addition to improvement of airway mucus. Based on this finding, we assumed that, if herb water containing 1,8−cineol exhibits a bacteriostatic effect in addition to a fungistatic effect in children
and adults with severe psychosomatic disorders with difficulty in assisted brushing, it may exhibit a preventive effect against opportunistic infection, periodontal disease, and caries.
To clarify this, we investigated the bacteriostatic effects on the pathogens of these diseases in vitro. In the procedure, Eucalyptus globulus was extracted by steam distillation, and culture media supplemented with herb water containing 0.2 mg/ml 1,8−cineol were prepared for culture of the following microorganisms : Pathogens of opportunistic infection : Candida albicans (JCM 1842 strain) and Kelebsiella pneumoniae (JCM 1662 strain) ; pathogens of periodontal disease : Prevotella intermedia (ATCC 25611 strain ) , Prevotella nigrescens (ATCC 335663 strain), Actinobacillus actinomycetemcomitans (JCM 8577 strain), and Porphyromonas gingivalis (ATCC 49417 strain) ; pathogens of caries : Streptococcus mutans (Ingbritt strain) and Streptococcus sobrinus (6715 strain). These were cultured aerobically or anerobically, and the bacteriostatic effect was investigated employing turbidimetry. In addition, the effect on Streptococcus salivarius (JCM 5707 strain) was investigated to clarify the influence on oral indigenous bacteria. The following findings were  obtained : 1. Herb water containing 1,8−cineol exhibited bacterio− and fungistatic effects on the pathogens of opportunistic infection, periodontal disease, and severe caries.
2. Herb water containing 1,8−cineol exhibited no bacteriostatic effect on an oral indigenous bacterium, Streptococcus salivarius. It was suggested that herb water containing 1,8− cineol may be applicable for the prevention of opportunistic infection, periodontal disease, and caries without destroying the oral indigenous bacterial flora in children and adults with severe psychosomatic disorders accompanied by eating and swallowing disorders due to an unstable systemic condition, in whom assisted brushing is difficult.},
 pages = {12--21},
 title = {ユーカリ葉抽出物による日和見感染，歯周疾患，う蝕予防についての検討　重症心身障害児・者への口腔ケアのために},
 volume = {38},
 year = {2012}
}