{"created":"2023-06-19T07:38:40.974317+00:00","id":1824,"links":{},"metadata":{"_buckets":{"deposit":"b2cf466a-cde3-4976-9d46-c90a173f4e22"},"_deposit":{"created_by":3,"id":"1824","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"1824"},"status":"published"},"_oai":{"id":"oai:mdu.repo.nii.ac.jp:00001824","sets":["1:173:180"]},"author_link":["687","686","685","684","688","689"],"item_2_alternative_title_1":{"attribute_name":"その他（別言語等）のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"Porphyromonas gingivalis ４株における病原性関連酵素とタンパクの比較研究"}]},"item_2_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2013-12-31","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"130","bibliographicPageStart":"126","bibliographicVolumeNumber":"39","bibliographic_titles":[{"bibliographic_title":"松本歯学"}]}]},"item_2_description_19":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_2_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Porphyromonas gingivalis の研究上使われる代表的な４株（ATCC３３２７７，３８１，W５０，W８３）を用いて，病原因子と深い関係のある，RGP，KGP を含むプロテイナーゼ，ペプチダーゼおよびヘモグロビンとミオグロビン結合タンパクを通しての結合活性について比較検討した．プロテイナーゼに関して，４株ともにその産生が見られ，それぞれの株で３画分での分布状況を調べたところ，RGP，KGP，アゾコル分解酵素ともに，培養上清，粗抽出液，エンベロープの順に多かった．RGP，KGP に関しては，ATCC３３２７７，３８１，W５０で培養上清にほぼ６０％ほど含まれていたが，W８３では９４％を含んでいた．これに反して，ペプチダーゼ（PTP，DPP−I，DPP−II，DPP−IV）は４株ともに培養上清には検出されず，そのほとんどが粗抽出液に認められ，数％のみがエンベロープに分布しており，ペプチダーゼには細胞外への分泌機構が作用しないものと思われる．いずれの株でもPTP 産生単位数がDPP のどれよりも高かった．菌体細胞のヘムタンパクへの結合もいずれの株でも見られたが，ともにpH 依存性が見られ，酸性で強く，中性，アルカリでは非常に弱かった．結合の度合いはヘモグロビンとミオグロビンでは違いはほとんど認められず，これはミオグロビンがヘモグロビン分子の四分の一から成っていることから当然と言えよう．他のヘムタンパク（チトクロームC，カタラーゼ）への結合も弱いながら観察された．","subitem_description_type":"Abstract"}]},"item_2_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"松本歯科大学学会"}]},"item_2_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00232590","subitem_source_identifier_type":"NCID"}]},"item_2_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0385-1613","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"KISO, AKIKO"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"MATSUO, KAZUKO"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"ONOZAWA, SATOSU"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"SHIBATA, YUKINAGA"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"HASEGAWA, HIROMASA"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"FUJIMURA, SETSUO"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2014-02-28"}],"displaytype":"detail","filename":"matsumoto_shigaku39-2-06.pdf","filesize":[{"value":"828.5 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"matsumoto_shigaku39-2-06.pdf","url":"https://mdu.repo.nii.ac.jp/record/1824/files/matsumoto_shigaku39-2-06.pdf"},"version_id":"3f22d152-735b-42e1-befc-795f42ebe6d6"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"proteinase","subitem_subject_scheme":"Other"},{"subitem_subject":"peptidase","subitem_subject_scheme":"Other"},{"subitem_subject":"binding","subitem_subject_scheme":"Other"},{"subitem_subject":"Porphyromonas gingivalis","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Comparative studies on the formation of pathogenicity related enzymes and protein among four strains of Porphyromonas gingivalis","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Comparative studies on the formation of pathogenicity related enzymes and protein among four strains of Porphyromonas gingivalis","subitem_title_language":"en"}]},"item_type_id":"2","owner":"3","path":["180"],"pubdate":{"attribute_name":"公開日","attribute_value":"2014-02-28"},"publish_date":"2014-02-28","publish_status":"0","recid":"1824","relation_version_is_last":true,"title":["Comparative studies on the formation of pathogenicity related enzymes and protein among four strains of Porphyromonas gingivalis"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-06-19T08:07:31.375607+00:00"}