{"created":"2023-06-19T07:38:43.798178+00:00","id":2242,"links":{},"metadata":{"_buckets":{"deposit":"1a0b44b2-ac8b-4b80-8cd1-40105d5f04eb"},"_deposit":{"created_by":3,"id":"2242","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"2242"},"status":"published"},"_oai":{"id":"oai:mdu.repo.nii.ac.jp:00002242","sets":["170:177"]},"author_link":["2179","2175","2176","2182","2180","2177","2178","2181"],"item_10006_alternative_title_1":{"attribute_name":"その他（別言語等）のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"マウス破骨細胞形成における抗微生物ペプチド Cathelicidin-related antimicrobial peptide （CRAMP）の役割）"}]},"item_10006_biblio_info_28":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2013-11-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"3","bibliographicPageEnd":"351","bibliographicPageStart":"344","bibliographicVolumeNumber":"140","bibliographic_titles":[{},{"bibliographic_title":"Immunology","bibliographic_titleLang":"en"}]}]},"item_10006_date_granted_11":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2014-03-06"}]},"item_10006_degree_grantor_9":{"attribute_name":"学位授与大学","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_name":"松本歯科大学"}]}]},"item_10006_degree_name_8":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士（歯学）"}]},"item_10006_description_10":{"attribute_name":"学位授与年度","attribute_value_mlt":[{"subitem_description":"2013","subitem_description_type":"Other"}]},"item_10006_description_12":{"attribute_name":"報告番号","attribute_value_mlt":[{"subitem_description":"甲第157号","subitem_description_type":"Other"}]},"item_10006_description_17":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_10006_description_7":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Cathelicidin-related antimicrobial peptide (CRAMP) not only kills bacteria but also binds to lipopolysaccharide (LPS) to neutralize its activity. CRAMP is highly expressed in bone marrow and its expression is reported to be up-regulated by inflammatory and infectious stimuli. Here, we examined the role of CRAMP in murine osteoclastogenesis. Osteoclasts were formed in co-cultures of osteoblasts and bone marrow cells in response to 1a,25-dihydroxyvitamin D3 [1a,25(OH)2D3], prostaglandin E2(PGE2), and Toll-like receptor (TLR) ligands such as LPS and flagellin through the induction of receptor activator of nuclear factor-jB ligand(RANKL) expression in osteoblasts. CRAMP inhibited the osteoclastogenesis in co-cultures treated with LPS and flagellin, but not in those treated with 1a,25(OH)2D3 or PGE2. Although bone marrow macrophages(BMMs) highly expressed formyl peptide receptor 2 (a receptor of CRAMP), CRAMP showed no inhibitory effect on osteoclastogenesis in BMM cultures treated with RANKL. CRAMP suppressed both LPS- and flagellin-induced RANKL expression in osteoblasts and tumour necrosis factor-a (TNF-a) expression in BMMs, suggesting that CRAMP neutralizes the actions of LPS and flagellin. LPS and flagellin enhanced the expression of CRAMP mRNA in osteoblasts. Extracellularly added CRAMP suppressed LPS- and flagellin-induced CRAMP expression. These results suggest that the production of CRAMP promoted by LPS and flagellin is inhibited by CRAMP released by osteoblasts through a feedback regulation. Even though CRAMP itself has no effect on osteoclastogenesis in mice, we propose that CRAMP is an osteoblast-derived protector in bacterial infection-induced osteoclastic bone resorption.","subitem_description_type":"Abstract"}]},"item_10006_dissertation_number_22":{"attribute_name":"学位授与番号","attribute_value_mlt":[{"subitem_dissertationnumber":"33602甲第157号"}]},"item_10006_relation_14":{"attribute_name":"情報源","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"雑誌掲載論文(電子版)"}]}]},"item_10006_relation_15":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"Wiley online library"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"http://onlinelibrary.wiley.com/maintenance/?DOI=10.1111/imm.12146","subitem_relation_type_select":"URI"}}]},"item_10006_relation_23":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"info:pmid/23826736 ","subitem_relation_type_select":"PMID"}}]},"item_10006_relation_24":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"info:doi/10.1111/imm.12146"}],"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1111/imm.12146","subitem_relation_type_select":"DOI"}}]},"item_10006_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© 2013 John Wiley & Sons Ltd"}]},"item_10006_source_id_27":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1365-2567","subitem_source_identifier_type":"ISSN"}]},"item_10006_version_type_18":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Horibe, Kanji"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nakamichi, Yuko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Shunsuke, Uehara"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nakamura, Midori"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Koide, Masanori"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kobayashi, Yasuhiro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Takahashi, Naoyuki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Udagawa, Nobuyuki"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2014-07-22"}],"displaytype":"simple","filename":"33602_A000184_shinsa-yoshi.pdf","filesize":[{"value":"332.1 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"内容の要旨および審査の結果の要旨","url":"https://mdu.repo.nii.ac.jp/record/2242/files/33602_A000184_shinsa-yoshi.pdf"},"version_id":"d3ef9da3-363c-418e-98f2-43e9b00179d8"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-10-14"}],"displaytype":"simple","filename":"33602_A000184_yoshi.pdf","filesize":[{"value":"131.8 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"学位論文の要旨","url":"https://mdu.repo.nii.ac.jp/record/2242/files/33602_A000184_yoshi.pdf"},"version_id":"311bc6dc-cba7-4ffd-8a30-2beb9b35e3f1"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"cathelicidin-related antimicrobial peptide","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"flagellin","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"lipopolysaccharide","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"osteoclastogenesis","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"receptor activator of nuclear factor-jB ligand","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"thesis","resourceuri":"http://purl.org/coar/resource_type/c_46ec"}]},"item_title":"Roles of Cathelicidin-related Antimicrobial Peptide in Murine Osteoclastogenesis","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Roles of Cathelicidin-related Antimicrobial Peptide in Murine Osteoclastogenesis","subitem_title_language":"en"}]},"item_type_id":"10006","owner":"3","path":["177"],"pubdate":{"attribute_name":"公開日","attribute_value":"2014-07-15"},"publish_date":"2014-07-15","publish_status":"0","recid":"2242","relation_version_is_last":true,"title":["Roles of Cathelicidin-related Antimicrobial Peptide in Murine Osteoclastogenesis"],"weko_creator_id":"3","weko_shared_id":3},"updated":"2023-06-19T07:56:46.358155+00:00"}