{"created":"2023-06-19T07:38:44.348009+00:00","id":2266,"links":{},"metadata":{"_buckets":{"deposit":"b7d789b3-9739-427c-b438-4b97a144b2bb"},"_deposit":{"created_by":3,"id":"2266","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"2266"},"status":"published"},"_oai":{"id":"oai:mdu.repo.nii.ac.jp:00002266","sets":["1:198:199"]},"author_link":["2290","2284","2285","2282","2287","2281","2289","2283","2286","2288"],"item_10001_alternative_title_1":{"attribute_name":"その他（別言語等）のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"Porphyromonas gingivalis のプロリルトリペプチジルペプチダーゼの産生，単離および性状"}]},"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2014-06-30","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"12","bibliographicPageStart":"1","bibliographicVolumeNumber":"40","bibliographic_titles":[{"bibliographic_title":"松本歯学"},{"bibliographic_title":"Journal of the Matsumoto Dental University Society","bibliographic_titleLang":"en"}]}]},"item_10001_creator_3":{"attribute_name":"著者別名","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"松尾, 和子"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"木曽, 有紀子"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"柴田, 幸永"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"長谷川, 博雅"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"藤村, 節夫"}],"nameIdentifiers":[{}]}]},"item_10001_description_19":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Formation, cellular locations, isolation and enzymatic properties of PTP of Porphyromonas\ngingivalis, an anaerobic periodontal pathogen were investigated. Almost all activities of this enzyme were detected in the de extract of the cell, but the other bacterial fractions such as culture fluids, envelopes and vesicles were not found to contain PTP. PTP was purified from the crude extract prepared by sonication and centrifugation through five steps including concentration, ion exchange hromatography, gel filtration, hydrophobic interaction chromatography and isoelectric focusing to homogeneity. The enzyme was a serine enzyme since it was inhibited strongly by Pefabloc SC, propyl\nfluorophosphate and 3,4–dichloroisocoumarin. It hydrolyzed H–Ala–Ala–Pro–pNA and H–Ala–Phe–Pro–pNA. The molecular mass was determined as 45 kDa and isoelectric point was 5.₇. Optimum pH was moderately broad, and maximum activity was observed in the range of pH ₇.0 to 9.0. The residual activity after heating at 50℃ for 5 min was 29%, but heating at 60℃ resulted\nin complete loss of the activity.","subitem_description_type":"Abstract"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"松本歯科大学学会"}]},"item_10001_relation_12":{"attribute_name":"NII論文ID","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"120005487653","subitem_relation_type_select":"NAID"}}]},"item_10001_relation_16":{"attribute_name":"情報源","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"博士学位論文(2014年度松本歯科大学大学院歯学独立研究科)"}]}]},"item_10001_relation_24":{"attribute_name":"医中誌ID","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"2015021011","subitem_relation_type_select":"ICHUSHI"}}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0385-1613","subitem_source_identifier_type":"ISSN"}]},"item_10001_text_25":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_text_value":"松本歯科大学大学院歯学独立研究科博士（歯学）学位申請論文 ; 大学院歯学独立研究科　硬組織疾患病態解析学（主指導教員：長谷川博雅）"}]},"item_10001_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"MATSUO, KAZUKO"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"KISO, AKIKO"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"SHIBATA, YUKINAGA"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"HASEGAWA, HIROMASA"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"FUJIMURA, SETSUO"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2014-09-11"}],"displaytype":"detail","filename":"matsumoto_shigaku40-1-01.pdf","filesize":[{"value":"2.2 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"matsumoto_shigaku40-1-01.pdf","url":"https://mdu.repo.nii.ac.jp/record/2266/files/matsumoto_shigaku40-1-01.pdf"},"version_id":"def0b16c-cadc-4968-9016-4424179b8ebb"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"enzyme","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"prolyl tripeptidase","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Porphyromonas gingivalis","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Formation, isolation and characterization of a prolyl tripeptidyl peptidase of Porphyromonas gingivalis","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Formation, isolation and characterization of a prolyl tripeptidyl peptidase of Porphyromonas gingivalis","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"3","path":["199"],"pubdate":{"attribute_name":"公開日","attribute_value":"2014-09-11"},"publish_date":"2014-09-11","publish_status":"0","recid":"2266","relation_version_is_last":true,"title":["Formation, isolation and characterization of a prolyl tripeptidyl peptidase of Porphyromonas gingivalis"],"weko_creator_id":"3","weko_shared_id":3},"updated":"2023-06-19T08:01:37.882145+00:00"}