@article{oai:mdu.repo.nii.ac.jp:00002268,
 author = {小町谷, 美帆 and 溝上, 真也 and 三原, 正志 and 大澤, 雅樹 and 菊池, 有一郎 and 上松, 節子 and 平井, 要 and 大久保, 裕一郎 and 黒岩, 昭弘 and 山田, 一尋 and 山倉, 文幸 and 平岡, 行博 and KOMACHIYA, MIHO and MIZOUE, SHINYA and MIHARA, MASASHI and OSAWA, MASAKI and KIKUCHI, YUICHIRO and UEMATSU, SETSUKO and UEMATSU, SETSUKO and OKUBO, YUICHIRO and KUROIWA, AKIHIRO and YAMADA, KAZUHIRO and YAMAKURA, FUMIYUKI and HIRAOKA, B.YUKIHIRO},
 issue = {1},
 journal = {松本歯学, Journal of the Matsumoto Dental University Society},
 month = {Jun},
 note = {application/pdf, Porphyromonas gingivalis contains a single constitutive superoxide dismutase (SOD) that is active with either iron or manganese at the active site. The aim of this work was to evaluate the effect of the Leu ₇2 to Trp mutation on the structure of P. gingivalis SOD (Pg SOD) using lectrophoretic characterization. Leu ₇2, which is located near the active site metal, is substituted with Trp in aligned amino acid sequences of iron–containing SOD. The results of electrophoretic characterization and the expressed activity of mutant SOD suggest that mutant SOD have the same gross structure as wild–type SOD. We herein conclude that the integrity of Leu ₇2 is a necessary requisite for the metal–tolerant activity of Pg SOD.},
 pages = {19--25},
 title = {Effect of substituting Trp for Leu at position 72 on the structure of Porphyromonas gingivalis superoxide dismutase},
 volume = {40},
 year = {2014}
}