{"created":"2023-06-19T07:38:44.558741+00:00","id":2269,"links":{},"metadata":{"_buckets":{"deposit":"8559e46f-d911-4be1-9c01-e89d3d82ae41"},"_deposit":{"created_by":3,"id":"2269","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"2269"},"status":"published"},"_oai":{"id":"oai:mdu.repo.nii.ac.jp:00002269","sets":["1:198:199"]},"author_link":["2331","2339","2337","2325","2329","2340","2348","2347","2334","2332","2342","2326","2345","2344","2346","2335","2330","2336","2341","2333","2343","2327","2338","2328"],"item_10001_alternative_title_1":{"attribute_name":"その他（別言語等）のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"Porphyromonas gingivalis SOD における活性中心近傍に局在するアミノ酸残基の金属特異的活性における関与：Leu ₇2 Trp およびLeu ₇6 Phe の2残基変異による検討"}]},"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2014-06-30","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"34","bibliographicPageStart":"26","bibliographicVolumeNumber":"40","bibliographic_titles":[{"bibliographic_title":"松本歯学"},{"bibliographic_title":"Journal of the Matsumoto Dental University Society","bibliographic_titleLang":"en"}]}]},"item_10001_creator_3":{"attribute_name":"著者別名","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"三原, 正志"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"小町谷, 美帆"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"溝上, 真也"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"大澤, 雅樹"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"上松, 節子"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"菊池, 有一郎"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"大久保, 裕一郎"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"平井, 要"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"黒岩, 昭弘"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"山田, 一尋"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"山倉, 文幸"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"平岡, 行博"}],"nameIdentifiers":[{}]}]},"item_10001_description_19":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The role of superoxide dismutase (SOD) as a radical scavenger in Porphyromonas gingivalis is well documented. P. gingivalis SOD (Pg SOD), which is characterized by a metal–tolerant activity, can use either iron or manganese as a cofactor. Leu ₇2 and Leu ₇6, located near the active–site metal, are characteristic amino acid sequences of Pg SOD proteins, although they are substituted to Trp in the ₇2 position and Phe in the ₇6 position in most iron–containing SOD (Fe–SOD) proteins. In the present study, we constructed a mutant of the enzyme with changes from Leu ₇2 to Trp and Leu ₇6 to Phe. This mutant SOD was examined with respect to its metal–dependent activity and structural characterization. We herein conclude the integrity of Leu ₇2 and Leu ₇6 is a necessary requisite for the metal–tolerant activity of Pg SOD.","subitem_description_type":"Abstract"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"松本歯科大学学会"}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0385-1613","subitem_source_identifier_type":"ISSN"}]},"item_10001_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"MIHARA, MASASHI"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"KOMACHIYA, MIHO"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"MIZOUE, SHINYA"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"OSAWA, MASAKI"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"UEMATSU, SETSUKO"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"KIKUCHI, YUICHIRO"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"OKUBO, YUICHIRO"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"HIRAI, KANAME"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"KUROIWA, AKIHIRO"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"YAMADA, KAZUHIRO"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"YAMAKURA, FUMIYUKI"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"HIRAOKA, B.YUKIHIRO"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2014-09-11"}],"displaytype":"detail","filename":"matsumoto_shigaku40-1-04.pdf","filesize":[{"value":"1.7 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"matsumoto_shigaku40-1-04.pdf","url":"https://mdu.repo.nii.ac.jp/record/2269/files/matsumoto_shigaku40-1-04.pdf"},"version_id":"d7f876ad-8a23-4c34-97d5-2142859a6edd"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"site–directed mutagenesis","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"superoxide dismutase","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Porphyromonas gingivalis","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Contribution of the amino acid residues located near the active site metal to the metal–specific activity of Porphyromonas gingivalis SOD induced by a double mutation of Leu 72 Trp and Leu 76 Phe","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Contribution of the amino acid residues located near the active site metal to the metal–specific activity of Porphyromonas gingivalis SOD induced by a double mutation of Leu 72 Trp and Leu 76 Phe","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"3","path":["199"],"pubdate":{"attribute_name":"公開日","attribute_value":"2014-09-11"},"publish_date":"2014-09-11","publish_status":"0","recid":"2269","relation_version_is_last":true,"title":["Contribution of the amino acid residues located near the active site metal to the metal–specific activity of Porphyromonas gingivalis SOD induced by a double mutation of Leu 72 Trp and Leu 76 Phe"],"weko_creator_id":"3","weko_shared_id":3},"updated":"2023-06-19T08:06:32.371078+00:00"}