{"created":"2023-06-19T07:38:45.397591+00:00","id":2281,"links":{},"metadata":{"_buckets":{"deposit":"f990c9a6-7c00-4b52-a40d-b35a5c638c5a"},"_deposit":{"created_by":3,"id":"2281","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"2281"},"status":"published"},"_oai":{"id":"oai:mdu.repo.nii.ac.jp:00002281","sets":["178:204","193:194:195","193:205:273","193:206:277","193:208:276","193:235:270"]},"author_link":["2383","2382","2381","2379","2380"],"item_10001_alternative_title_1":{"attribute_name":"その他（別言語等）のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"リポ多糖類処理を行ったヒト歯肉線維芽細胞の炎症反応に対する漢方薬半夏瀉心湯の予防効果"}]},"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2010-03-10","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"50","bibliographicPageStart":"43","bibliographicVolumeNumber":"19","bibliographic_titles":[{},{"bibliographic_title":"Journal of Hard Tissue Biology","bibliographic_titleLang":"en"}]}]},"item_10001_description_19":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"In the present study, we investigated the effects of a Kampo medicine hangeshashinto (TJ-14) on the production of prostaglandin E2 (PGE2), interleukin (IL)-6 and IL-8 by human gingival fibroblasts (HGFs) treated with lipopolysaccharide (LPS) from Porphyromonas gingivalis. HGFs proliferation was dose-dependently decreased with hangeshashinto at days 3 and 7. However, treatment with LPS (10 ng/ml), hangeshashinto (up to 1 mg/ml) and their combinations for 24 h did not affect the viability of HGFs. Hangeshashinto dose-dependently suppressed LPS-induced PGE2 production but did not alter basal PGE2 level. Hangeshashinto weakly decreased LPS-induced IL-6 and IL-8 productions. Hangeshashinto decreased cyclooxygenase (COX)-1 and COX-2 activities to approximately 60% at 1 mg/ml. Hangeshashinto decreased cytoplasmic phospholipase A2 (cPLA2) expression and LPS-induced COX-2 expression but not affected annexin1 expression. Hangeshashinto weakly suppressed LPS-induced extracellular signal-regulated kinase (ERK) phosphorylation, which is known to lead to ERK activation and cPLA2 phosphorylation. These results suggest that hangeshashinto decreased PGE2 production by (1) suppression of cPLA2 and LPS-induced COX-2 expression, and to a lesser extent, (2) inhibition of COX-2 activity and (3) inhibition of cPLA2 phosphorylation and its activation via inhibition of ERK phosphorylation. Moreover, it is also suggested that hangeshashinto may be useful to improve gingival inflammation in periodontal disease.","subitem_description_type":"Abstract"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"The Hard Tissue Biology Network Association(JHTBNet)"}]},"item_10001_relation_12":{"attribute_name":"NII論文ID","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10030688335","subitem_relation_type_select":"NAID"}}]},"item_10001_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"info:doi/10.2485/jhtb.19.43"}],"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.2485/jhtb.19.43","subitem_relation_type_select":"DOI"}}]},"item_10001_relation_17":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"J-STAGE"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"http://doi.org/10.2485/jhtb.19.43","subitem_relation_type_select":"DOI"}}]},"item_10001_relation_18":{"attribute_name":"他の資源との関係","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"2010年度 松本歯科大学大学院歯学独立研究科 博士学位論文（歯学）：33602乙第17号"}]}]},"item_10001_relation_24":{"attribute_name":"医中誌ID","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"2011052693","subitem_relation_type_select":"ICHUSHI"}}]},"item_10001_rights_15":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"Copyright © 2010 by The Hard Tissue Biology Network Association(JHTBNet) "}]},"item_10001_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA11074332","subitem_source_identifier_type":"NCID"}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1880-828X","subitem_source_identifier_type":"ISSN"}]},"item_10001_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"NAKAZONO, YODAI"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"ARA, TOSHIAKI"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"FUJINAMI, YOSHIAKI"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"HATTORI, TOSHIMI"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"WANG, PAO-LI"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2014-10-06"}],"displaytype":"detail","filename":"2014-006_JA.pdf","filesize":[{"value":"239.8 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"2014-006_JA.pdf","url":"https://mdu.repo.nii.ac.jp/record/2281/files/2014-006_JA.pdf"},"version_id":"01973518-2f6a-4533-b340-2f20da6ca599"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Anti-inflammatory effect","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Hangeshashinto","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Human gingival fibroblast","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Prostaglandin E2","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Preventive Effects of a Kampo Medicine, Hangeshashinto on Inflammatory Responses in ipopolysaccharide-Treated Human Gingival Fibroblasts","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Preventive Effects of a Kampo Medicine, Hangeshashinto on Inflammatory Responses in ipopolysaccharide-Treated Human Gingival Fibroblasts","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"3","path":["195","204","270","273","276","277"],"pubdate":{"attribute_name":"公開日","attribute_value":"2014-10-06"},"publish_date":"2014-10-06","publish_status":"0","recid":"2281","relation_version_is_last":true,"title":["Preventive Effects of a Kampo Medicine, Hangeshashinto on Inflammatory Responses in ipopolysaccharide-Treated Human Gingival Fibroblasts"],"weko_creator_id":"3","weko_shared_id":3},"updated":"2023-06-19T07:59:20.484950+00:00"}