{"created":"2023-06-19T07:38:45.467289+00:00","id":2282,"links":{},"metadata":{"_buckets":{"deposit":"c23bc8bb-d6fa-4510-a6b8-503354c425e4"},"_deposit":{"created_by":3,"id":"2282","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"2282"},"status":"published"},"_oai":{"id":"oai:mdu.repo.nii.ac.jp:00002282","sets":["178:187","193:194:195","193:205:272","193:205:273","193:233:274","193:234:271","193:235:270"]},"author_link":["2384","2386","2385","2389","2387","2388"],"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2012","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"11","bibliographicVolumeNumber":"10","bibliographic_titles":[{},{"bibliographic_title":"Journal of Negative Results in BioMedicine","bibliographic_titleLang":"en"}]}]},"item_10001_description_19":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Objective: Periodontal disease is accompanied by inflammation of the gingiva and destruction of periodontal\ntissues, leading to alveolar bone loss in severe clinical cases. Interleukin (IL)-6, IL-8, and the chemical mediator\nprostaglandin E2 (PGE2) are known to play important roles in inflammatory responses and tissue degradation.\nRecently, we reported that the protein kinase A (PKA) inhibitor H-89 suppresses lipopolysaccharide (LPS)-induced\nIL-8 production by human gingival fibroblasts (HGFs). In the present study, the relevance of the PKA activity and\ntwo PKA-activating drugs, aminophylline and adrenaline, to LPS-induced inflammatory cytokines (IL-6 and IL-8) and\nPGE2 by HGFs were examined.\nMethods: HGFs were treated with LPS from Porphyromonas gingivalis and H-89, the cAMP analog dibutyryl cyclic\nAMP (dbcAMP), aminophylline, or adrenaline. After 24 h, IL-6, IL-8, and PGE2 levels were evaluated by ELISA.\nResults: H-89 did not affect LPS-induced IL-6 production, but suppressed IL-8 and PGE2 production. In contrast,\ndbcAMP significantly increased LPS-induced IL-6, IL-8, and PGE2 production. Up to 10 μg/ml of aminophylline did\nnot affect LPS-induced IL-6, IL-8, or PGE2 production, but they were significantly increased at 100 μg/ml. Similarly,\n0.01 μg/ml of adrenaline did not affect LPS-induced IL-6, IL-8, or PGE2 production, but they were significantly\nincreased at concentrations of 0.1 and 1 μg/ml. In the absence of LPS, H-89, dbcAMP, aminophylline, and\nadrenaline had no relevance to IL-6, IL-8, or PGE2 production.\nConclusion: These results suggest that the PKA pathway, and also PKA-activating drugs, enhance LPS-induced IL-6,\nIL-8, and PGE2 production by HGFs. However, aminophylline may not have an effect on the production of these\nmolecules at concentrations used in clinical settings (8 to 20 μg/ml in serum). These results suggest that\naminophylline does not affect inflammatory responses in periodontal disease.","subitem_description_type":"Abstract"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"BioMed Central"}]},"item_10001_relation_13":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"info:pmid/22452847","subitem_relation_type_select":"PMID"}}]},"item_10001_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"info:doi/10.1186/1477-5751-11-10."}],"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1186/1477-5751-11-10.","subitem_relation_type_select":"DOI"}}]},"item_10001_relation_16":{"attribute_name":"情報源","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"雑誌掲載論文(電子版)"}]}]},"item_10001_relation_17":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"BioMed Central Ltd"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"http://www.jnrbm.com/content/11/1/10","subitem_relation_type_select":"URI"}}]},"item_10001_rights_15":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© 2012 Ara et al; licensee BioMed Central Ltd. "}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1477-5751","subitem_source_identifier_type":"ISSN"}]},"item_10001_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"ARA, TOSHIAKI"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"FUJINAMI, YOSHIAKI"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"URANO, HIROKO"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"HIRAI, KANAME"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"HATTORI, TOSHIMI"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"MIYAZAWA, HIROO"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2014-10-06"}],"displaytype":"detail","filename":"2014-002_JA.pdf","filesize":[{"value":"1.6 MB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"2014-002_JA.pdf","url":"https://mdu.repo.nii.ac.jp/record/2282/files/2014-002_JA.pdf"},"version_id":"749d9782-e529-45a4-9cc7-d930a268c744"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Protein kinase A","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"interleukin,","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"prostaglandin E2","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"human gingival fibroblast","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Cyclic AMP-Dependent Protein Kinases","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Dinoprostone","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Fibroblasts","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Gingiva","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Interleukin-6","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Interleukin-8","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Lipopolysaccharides","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Protein kinase A enhances lipopolysaccharideinduced IL-6, IL-8, and PGE2 production by human gingival broblasts","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Protein kinase A enhances lipopolysaccharideinduced IL-6, IL-8, and PGE2 production by human gingival broblasts","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"3","path":["187","195","270","271","272","273","274"],"pubdate":{"attribute_name":"公開日","attribute_value":"2014-10-06"},"publish_date":"2014-10-06","publish_status":"0","recid":"2282","relation_version_is_last":true,"title":["Protein kinase A enhances lipopolysaccharideinduced IL-6, IL-8, and PGE2 production by human gingival broblasts"],"weko_creator_id":"3","weko_shared_id":3},"updated":"2023-06-19T07:59:12.412726+00:00"}