@article{oai:mdu.repo.nii.ac.jp:00000232,
 author = {佐原, 紀行 and 赤羽, 章司},
 issue = {2},
 journal = {松本歯学},
 month = {Dec},
 note = {application/pdf, A variety of procedures have been employed to prepare soft tissues and cells to observe their intracellar stractures by the scanning electron microscope. However, their procedures often unsatisfactory because of artifact caused by shearing forces and other reasons. The present study attempt to examine successful procedure for intracellar stractures of mouse submandibular glands. After cryofracture was used, mild ion-etching (700V) or low-pH, hypotonic solution (without ion-etching) method was applicated for disclose intracellar stractures of their cells. Ion-etching specimens were examined by three diffent fixations and effect of ionetching time. Specimens fixed with paraformaldehyde and glutaraldehyde resulted in better ionetching effect than those fixed with osmium tetroxide. Successuful result for disclose intracellar stractures was obtained from paraformaldehyde and glutaraldehyde fixation, and ion-etching specimens for 10-15 minutes. Without ion-etching method did not get good results in submandibular glands in present study.},
 pages = {181--190},
 title = {走査電子顕微鏡によるマウス顎下腺の細胞内構造の観察},
 volume = {5},
 year = {1979}
}