@article{oai:mdu.repo.nii.ac.jp:00000235, author = {菱田, 市和 and 山本, 一郎 and 小松, 正隆 and 平岡, 行博}, issue = {2}, journal = {松本歯学}, month = {Dec}, note = {application/pdf, The present study was aimed, firstly to apply the modified Marmur's method (by Hill) in extracting DNA from the cultured tumor cells, and secondly to investigate some properties of the extracted DNA. The cultured tumor cells had been taken from the rhabdomyosarcoma in rats induced by injecting DMBA, as reported before, and been kept in the Department of Oral Surgery II, Matsumoto Dental College. Five samples were taken from the 70th to 80th generation of the cultured cells. The DNA was extracted by employing the modified Marmur's method of Hill, which is excellent in obtaining the pure DNA. The extracted DNA was examined by the ultra-violet spectrograph, using the Hitachi Spectrophotometer Model 101, and weight measurements in the Burton's method. The ratio of the ultra-violet absorption at the 260nm wave length to that at 280nm varied little, with an average of 1.9, which coincided with the studies reported by other investigators. The mean weight measure was 100μg/ml, with slight deviation. These results might indicate that the modified Marmur's method of Hill is excellent for obtaining the pure DNA from the cultured tumor cells of rats.}, pages = {208--212}, title = {Marmur変法による株化腫瘍細胞DNAの抽出}, volume = {5}, year = {1979} }