{"created":"2023-06-19T07:38:48.456978+00:00","id":2408,"links":{},"metadata":{"_buckets":{"deposit":"b7b6b479-055a-4f47-8a47-f4dc5c84d900"},"_deposit":{"created_by":3,"id":"2408","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"2408"},"status":"published"},"_oai":{"id":"oai:mdu.repo.nii.ac.jp:00002408","sets":["170:218"]},"author_link":["3704","2711","2712","2713"],"item_10006_alternative_title_1":{"attribute_name":"その他（別言語等）のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"LPS 刺激ヒト歯肉線維芽細胞におけるExtracellular Signal-Regulated Kinase のリン酸化抑制を介した葛根湯の炎症反応抑制効果"}]},"item_10006_biblio_info_28":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2014-02-18","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"Article ID 784019","bibliographicVolumeNumber":"2014","bibliographic_titles":[{},{"bibliographic_title":"ISRN pharmacology","bibliographic_titleLang":"en"}]}]},"item_10006_creator_3":{"attribute_name":"著者別名","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"喜多村, 洋幸"}],"nameIdentifiers":[{}]}]},"item_10006_date_granted_11":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2015-02-05"}]},"item_10006_degree_grantor_9":{"attribute_name":"学位授与大学","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_name":"松本歯科大学"}],"subitem_degreegrantor_identifier":[{}]}]},"item_10006_degree_name_8":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士（歯学）"}]},"item_10006_description_10":{"attribute_name":"学位授与年度","attribute_value_mlt":[{"subitem_description":"2014","subitem_description_type":"Other"}]},"item_10006_description_12":{"attribute_name":"報告番号","attribute_value_mlt":[{"subitem_description":"甲第160号","subitem_description_type":"Other"}]},"item_10006_description_17":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_10006_description_7":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Periodontal disease is accompanied by inflammation of the gingiva and destruction of periodontal tissues, leading to alveolar bone loss in severe clinical cases. The chemical mediator prostaglandin E2 (PGE2) and cytokines such as interleukin- (IL-)6 and IL-8 have been known to play important roles in inflammatory responses and tissue degradation. In the present study, we investigated the effects of a kampo medicine, kakkonto (TJ-1), on the production of prostaglandin E2 (PGE2), IL-6, and IL-8 by human gingival fibroblasts (HGFs) treated with lipopolysaccharide (LPS) from Porphyromonas gingivalis. Kakkonto concentration dependently suppressed LPS-induced PGE2 production but did not alter basal PGE2 levels. In contrast, kakkonto significantly increased LPSinduced IL-6 and IL-8 production. Kakkonto decreased cyclooxygenase- (COX-)1 activity to approximately 70% at 1mg/mL but did not affect COX-2 activity. Kakkonto did not affect cytoplasmic phospholipase A2 (cPLA2), annexin1, or LPS-induced COX-2 expression. Kakkonto suppressed LPS-induced extracellular signal-regulated kinase (ERK) phosphorylation, which is known to lead to ERK activation and cPLA2 phosphorylation. These results suggest that kakkonto decreased PGE2 production by inhibition of ERK phosphorylation which leads to inhibition of cPLA2 phosphorylation and its activation. Therefore, kakkonto may be useful to improve gingival inflammation in periodontal disease. ","subitem_description_type":"Abstract"}]},"item_10006_dissertation_number_22":{"attribute_name":"学位授与番号","attribute_value_mlt":[{"subitem_dissertationnumber":"甲第160号"}]},"item_10006_relation_14":{"attribute_name":"情報源","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"雑誌掲載論文(電子版)"}]}]},"item_10006_relation_15":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"PubMed Central® (PMC)"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3945151/","subitem_relation_type_select":"URI"}}]},"item_10006_relation_23":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"info:pmid/24693448","subitem_relation_type_select":"PMID"}}]},"item_10006_relation_24":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"info:doi/10.1155/2014/784019"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1155/2014/784019","subitem_relation_type_select":"DOI"}}]},"item_10006_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"Copyright © 2014 Hiroyuki Kitamura et al. This is an open access article distributed under the Creative Commons Attribution License(https://creativecommons.org/licenses/by/3.0/)"}]},"item_10006_source_id_27":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"2090-5165 ","subitem_source_identifier_type":"ISSN"}]},"item_10006_version_type_18":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Kitamura,  Hiroyuki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Urano,  Hiroko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ara,  Toshiaki"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2015-07-15"}],"displaytype":"detail","filename":"33602_A000187_honbun.pdf","filesize":[{"value":"1.8 MB"}],"format":"application/pdf","licensetype":"license_6","mimetype":"application/pdf","url":{"label":"本文","url":"https://mdu.repo.nii.ac.jp/record/2408/files/33602_A000187_honbun.pdf"},"version_id":"dcbceb26-f4cf-45f2-b4ec-055182270980"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2015-07-15"}],"displaytype":"simple","filename":"33602_A000187_shinsa-yoshi.pdf","filesize":[{"value":"301.9 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"内容の要旨および審査の結果の要旨","url":"https://mdu.repo.nii.ac.jp/record/2408/files/33602_A000187_shinsa-yoshi.pdf"},"version_id":"bc0491c3-5d73-4148-be75-d57c39ad5921"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Anti-Inflammatory Agents","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Medicine Kampo","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Gingiva cytology","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"gingiva drug effects","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Lipopolysaccharide","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Kakkonto","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"Preventive effects of a Kampo Medicine, Kakkonto, on inflammatory responses via the suppression of extracellular Signal-Regulated Kinase Phosphorylation in Lipopolysaccharide-Treated human gingival fibroblasts","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Preventive effects of a Kampo Medicine, Kakkonto, on inflammatory responses via the suppression of extracellular Signal-Regulated Kinase Phosphorylation in Lipopolysaccharide-Treated human gingival fibroblasts","subitem_title_language":"en"}]},"item_type_id":"10006","owner":"3","path":["218"],"pubdate":{"attribute_name":"公開日","attribute_value":"2015-07-15"},"publish_date":"2015-07-15","publish_status":"0","recid":"2408","relation_version_is_last":true,"title":["Preventive effects of a Kampo Medicine, Kakkonto, on inflammatory responses via the suppression of extracellular Signal-Regulated Kinase Phosphorylation in Lipopolysaccharide-Treated human gingival fibroblasts"],"weko_creator_id":"3","weko_shared_id":3},"updated":"2023-06-19T07:56:49.980354+00:00"}