@article{oai:mdu.repo.nii.ac.jp:00002707,
 author = {IMAMURA, YASUHIRO and HONDA, YOSHITOMO and MASUNO, KAZUYA and NAKAMURA, HIROE and WANG, PAO-LI},
 issue = {2},
 journal = {Journal of Hard Tissue Biology},
 month = {Apr},
 note = {application/pdf, Porcine placenta extract (P-placenta) is widely applied in medicine and cosmetics. However, few studies have examined the effect of the extract on the cellular behavior of the osteoblastic cell line Saos-2. Here, we demonstrated that P-placenta enhances the proliferation, collagen type I production, and alkaline phosphatase (ALP) secretion of Saos-2 in vitro. Proliferation of Saos-2 was assessed by MTT and DNA synthesis
assays. Type I collagen production and ALP secretion were evaluated using enzyme-linked immunosorbent assay and ALP assays. The cells were treated with/without 20, 200 and 2000 g/ml of P-placenta for 24 h. We found that 200 g/ml P-placenta significantly induced the proliferation of Saos-2 and enhanced type I collagen production and ALP secretion. The results indicate that P-placenta controls the cellular behavior of osteoblasts,
resulting in the secretion of early bone-related biomarkers.},
 pages = {157--160},
 title = {Effects of Placental Extract on Cell Proliferation, Type I Collagen Production, and ALP Secretion in Human Osteosarcoma Cell Line Saos-2},
 volume = {26},
 year = {2017}
}