{"created":"2023-06-19T07:39:03.049065+00:00","id":2707,"links":{},"metadata":{"_buckets":{"deposit":"4846ff2c-069b-40cb-b9a1-da8d63bdb045"},"_deposit":{"created_by":3,"id":"2707","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"2707"},"status":"published"},"_oai":{"id":"oai:mdu.repo.nii.ac.jp:00002707","sets":["178:204","193:236:238"]},"author_link":["4026","4025","4028","4027","4029"],"item_10001_alternative_title_1":{"attribute_name":"その他（別言語等）のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"胎盤抽出物がヒト骨肉腫細胞株Saos-2の細胞増殖、I型コラーゲン産生およびALP分泌に及ぼす効果"}]},"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2017-04","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"160","bibliographicPageStart":"157","bibliographicVolumeNumber":"26","bibliographic_titles":[{},{"bibliographic_title":"Journal of Hard Tissue Biology","bibliographic_titleLang":"en"}]}]},"item_10001_description_19":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Porcine placenta extract (P-placenta) is widely applied in medicine and cosmetics. However, few studies have examined the effect of the extract on the cellular behavior of the osteoblastic cell line Saos-2. Here, we demonstrated that P-placenta enhances the proliferation, collagen type I production, and alkaline phosphatase (ALP) secretion of Saos-2 in vitro. Proliferation of Saos-2 was assessed by MTT and DNA synthesis\nassays. Type I collagen production and ALP secretion were evaluated using enzyme-linked immunosorbent assay and ALP assays. The cells were treated with/without 20, 200 and 2000 g/ml of P-placenta for 24 h. We found that 200 g/ml P-placenta significantly induced the proliferation of Saos-2 and enhanced type I collagen production and ALP secretion. The results indicate that P-placenta controls the cellular behavior of osteoblasts,\nresulting in the secretion of early bone-related biomarkers.","subitem_description_type":"Abstract"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Society for Hard Tissue Regenerative Biology (硬組織再生生物学会)"}]},"item_10001_relation_12":{"attribute_name":"NII論文ID","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"40021177219","subitem_relation_type_select":"NAID"}}]},"item_10001_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"info:doi/10.2485/jhtb.26.157"}],"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.2485/jhtb.26.157","subitem_relation_type_select":"DOI"}}]},"item_10001_relation_16":{"attribute_name":"情報源","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"雑誌掲載論文(電子版) "}]}]},"item_10001_relation_17":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"Jstage"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.2485/jhtb.26.157","subitem_relation_type_select":"DOI"}}]},"item_10001_relation_24":{"attribute_name":"医中誌ID","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"2018007458","subitem_relation_type_select":"ICHUSHI"}}]},"item_10001_rights_15":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© 2017 by The Hard Tissue Biology Network Association(JHTBNet) "}]},"item_10001_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA11074332","subitem_source_identifier_type":"NCID"}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1341-7649","subitem_source_identifier_type":"ISSN"}]},"item_10001_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"IMAMURA, YASUHIRO"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"HONDA, YOSHITOMO"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"MASUNO, KAZUYA"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"NAKAMURA, HIROE"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"WANG, PAO-LI"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-03-07"}],"displaytype":"detail","filename":"2017-ja-016.pdf","filesize":[{"value":"697.5 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"本文","url":"https://mdu.repo.nii.ac.jp/record/2707/files/2017-ja-016.pdf"},"version_id":"90f88e03-8287-4d60-bb4a-2db87bed4da7"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"骨肉腫","subitem_subject_scheme":"Other"},{"subitem_subject":"胎盤抽出物","subitem_subject_scheme":"Other"},{"subitem_subject":"細胞増殖","subitem_subject_scheme":"Other"},{"subitem_subject":"腫瘍細胞系","subitem_subject_scheme":"Other"},{"subitem_subject":"ヒト","subitem_subject_scheme":"Other"},{"subitem_subject":"ブタ","subitem_subject_scheme":"Other"},{"subitem_subject":"動物","subitem_subject_scheme":"Other"},{"subitem_subject":"ALP","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Collagen","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Osteoblast","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Placenta","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Saos-2","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Alkaline Phosphatase","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"ELISA","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Tetrazolium Salts","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Thiazoles","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Collagen Type I","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Thiazolyl Blue","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Effects of Placental Extract on Cell Proliferation, Type I Collagen Production, and ALP Secretion in Human Osteosarcoma Cell Line Saos-2","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Effects of Placental Extract on Cell Proliferation, Type I Collagen Production, and ALP Secretion in Human Osteosarcoma Cell Line Saos-2","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"3","path":["204","238"],"pubdate":{"attribute_name":"公開日","attribute_value":"2018-03-07"},"publish_date":"2018-03-07","publish_status":"0","recid":"2707","relation_version_is_last":true,"title":["Effects of Placental Extract on Cell Proliferation, Type I Collagen Production, and ALP Secretion in Human Osteosarcoma Cell Line Saos-2"],"weko_creator_id":"3","weko_shared_id":3},"updated":"2023-06-19T07:58:47.154394+00:00"}