{"created":"2023-06-19T07:39:07.578539+00:00","id":2784,"links":{},"metadata":{"_buckets":{"deposit":"ba05de29-d418-4918-a03d-6489d1179cb4"},"_deposit":{"created_by":3,"id":"2784","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"2784"},"status":"published"},"_oai":{"id":"oai:mdu.repo.nii.ac.jp:00002784","sets":["178:204","193:206:302","193:206:308","193:232:303","193:232:397","193:233:470","193:234:268","193:242:380"]},"author_link":["4564","4567","4562","4563","4565","4561","4566"],"item_10001_alternative_title_1":{"attribute_name":"その他（別言語等）のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"神経誘導因子Netrin-1は軟骨形成や骨形成においてBMPまたはNogginにより調節される"}]},"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2012-04","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"149","bibliographicPageStart":"141","bibliographicVolumeNumber":"21","bibliographic_titles":[{},{"bibliographic_title":"Journal of Hard Tissue Biology","bibliographic_titleLang":"en"}]}]},"item_10001_description_19":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"This is the first report describing neurogenic factor of Netrin-1 related to chondrogenesis or osteogenesis in a human cells. Netrin is a morphogenetic factor that induces a growth cone of an axial filament of the nervous system. However, the roles of Netrin in chondrogenesis or osteogenesis are not yet understood. We analyzed the relationship between Netrin and bone morphogenetic protein-2 (BMP-2) in chondrogenesis or osteogenesis, using a human chondrocyte-like cell line (USAC), which also retains multi-potency to differentiate into osteoblasts and adipocytes. Netrin-1 mRNA was decreased in USAC cells, though the expression was increased during osteogenic differentiation at the stage when osteocalcin mRNA were increased by BMP-2. Furthermore, inhibition of Netrin-1 gene increased Cbfa1 mRNA expression, and decreased Sox9 mRNA expression. We also found that Netrin-1was strongly expressed in immature chondrocytes of cartilage-like tissues that were formed in an exo vivo experiment with diffusion chambers. The se findings indicate that Netrin-1 and BMP-2 regulates in the stage dependent process of mesenchymal cell differentiation to chondrocytes or osteoblasts.","subitem_description_type":"Abstract"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Society for Hard Tissue Regenerative Biology (硬組織再生生物学会)"}]},"item_10001_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"info:doi/10.2485/jhtb.21.141"}],"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.2485/jhtb.21.141","subitem_relation_type_select":"DOI"}}]},"item_10001_relation_16":{"attribute_name":"情報源","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"雑誌掲載論文(電子版) "}]}]},"item_10001_relation_17":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"Jstage"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.2485/jhtb.21.141","subitem_relation_type_select":"DOI"}}]},"item_10001_relation_24":{"attribute_name":"医中誌ID","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"2013275751","subitem_relation_type_select":"ICHUSHI"}}]},"item_10001_rights_15":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© 2012 by The Hard Tissue Biology Network Association(JHTBNet) "}]},"item_10001_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA11074332","subitem_source_identifier_type":"NCID"}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1341-7649","subitem_source_identifier_type":"ISSN"}]},"item_10001_text_25":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_text_value":"骨芽細胞または脂肪細胞への分化多能を保持するヒト軟骨細胞様細胞系(USAC)を用い、軟骨形成または骨形成におけるNetrinと骨形成蛋白質-2(BMP-2)との関係を調べた。Netrin-1 mRNAはUSAC細胞中では減少するが、オステオカルシンmRNA濃度がBMPによって上昇する際の骨芽細胞分化時にNetrin-1 mRNAの発現が増加した。Netrin-1遺伝子を阻害すると、Cbfal mRNA発現は増加しSox9 mRNA発現は減少した。またNetrin-1は軟骨様組織の未成熟軟骨細胞において強く発現した。Netrin-1とBMP-2が、間葉細胞の軟骨細胞または骨芽細胞へ分化プロセスを制御すると考えた。"}]},"item_10001_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Yagami, Kimitoshi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Sadaoka, Sunao"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nakamura, Midori"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nakamura, Hiroshi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Maki, Shigeru"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yagasaki, Tadashi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Udagawa, Nobuyuki"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-02-20"}],"displaytype":"detail","filename":"2018-ja-003.pdf","filesize":[{"value":"554.8 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"本文","url":"https://mdu.repo.nii.ac.jp/record/2784/files/2018-ja-003.pdf"},"version_id":"3b93e7a6-3a69-4132-b5a2-3f6edd253fd5"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"骨形成","subitem_subject_scheme":"Other"},{"subitem_subject":"細胞系","subitem_subject_scheme":"Other"},{"subitem_subject":"細胞分化","subitem_subject_scheme":"Other"},{"subitem_subject":"発生遺伝子発現調節","subitem_subject_scheme":"Other"},{"subitem_subject":"BALB Cマウス","subitem_subject_scheme":"Other"},{"subitem_subject":"軟骨形成","subitem_subject_scheme":"Other"},{"subitem_subject":"SOX9転写因子","subitem_subject_scheme":"Other"},{"subitem_subject":"骨形成因子-2","subitem_subject_scheme":"Other"},{"subitem_subject":"ヒト","subitem_subject_scheme":"Other"},{"subitem_subject":"マウス","subitem_subject_scheme":"Other"},{"subitem_subject":"動物","subitem_subject_scheme":"Other"},{"subitem_subject":"オス","subitem_subject_scheme":"Other"},{"subitem_subject":"歯学\\n","subitem_subject_scheme":"Other"},{"subitem_subject":"Netrin-1","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"BMP-2","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Noggin","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Chondrogensis","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Osteogenesis","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Carrier Proteins","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Osteocalcin","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Core Binding Factor Alpha 1 Subunit","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Netrin-1","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Noggin Protein\\n","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Neural-inducing Factor Netrin-1 is Regulated in Chondrogenesis and Osteogenesis by BMP or Noggin","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Neural-inducing Factor Netrin-1 is Regulated in Chondrogenesis and Osteogenesis by BMP or Noggin","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"3","path":["204","268","302","303","308","380","397","470"],"pubdate":{"attribute_name":"公開日","attribute_value":"2019-02-20"},"publish_date":"2019-02-20","publish_status":"0","recid":"2784","relation_version_is_last":true,"title":["Neural-inducing Factor Netrin-1 is Regulated in Chondrogenesis and Osteogenesis by BMP or Noggin"],"weko_creator_id":"3","weko_shared_id":3},"updated":"2023-06-19T07:56:28.701527+00:00"}