{"created":"2023-06-19T07:39:17.917343+00:00","id":2976,"links":{},"metadata":{"_buckets":{"deposit":"d509d474-2392-4f89-bdf3-b61ef879ec2f"},"_deposit":{"created_by":3,"id":"2976","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"2976"},"status":"published"},"_oai":{"id":"oai:mdu.repo.nii.ac.jp:00002976","sets":["170:542"]},"author_link":["5411","5410"],"item_10006_alternative_title_1":{"attribute_name":"その他（別言語等）のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"骨再生治療を目的とした皮質骨由来間葉系間質細胞によるスフェロイドの凍結保存"}]},"item_10006_biblio_info_28":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2021","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"4","bibliographicPageEnd":"263","bibliographicPageStart":"253","bibliographicVolumeNumber":"27","bibliographic_titles":[{},{"bibliographic_title":"Tissue Engineering Part C:Methods","bibliographic_titleLang":"en"}]}]},"item_10006_creator_3":{"attribute_name":"著者別名","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Dong, Hongwei"}],"nameIdentifiers":[{}]}]},"item_10006_date_granted_11":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2021-07-14"}]},"item_10006_degree_grantor_9":{"attribute_name":"学位授与大学","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_name":"松本歯科大学"}],"subitem_degreegrantor_identifier":[{}]}]},"item_10006_degree_name_8":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士（歯学）"}]},"item_10006_description_10":{"attribute_name":"学位授与年度","attribute_value_mlt":[{"subitem_description":"2021","subitem_description_type":"Other"}]},"item_10006_description_12":{"attribute_name":"報告番号","attribute_value_mlt":[{"subitem_description":"甲第239号","subitem_description_type":"Other"}]},"item_10006_description_17":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_10006_description_7":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Spontaneously formed spheroids from mouse compact bone-derived mesenchymal stromal cells (CB-MSCs) possess enhanced stemness and superior plasticity. In this study,the effect of cryopreservation on viabil ity,stemness,and osteogenic differentiation capability of spontaneous CB-MSC spheroids were investigated.CB-MSCs were isolated from mouse femur and tibia. Spheroids were cryopreserved with various concentra tions of dimethyl sulfoxide (DMSO). After thawing,the number of living and dead cells was measured.The expression levels of stem cell markers and osteogenic marker genes were analyzed. The cryopreserved and noncryopreserved spheroids were transplanted in mice with a beta-tricalcium phosphate as a scaffold to evaluate the in vivo bone-forming capability.The percentage of living cells was highest when 5% DMSO was used as a cryoprotectant,confirmed by the number of dead cells.The expression of stem cell marker genes and osteogenic differentiation capability were maintained after cryopreservation with 5% DMSO. The cryopre served spontaneous CB-MSC spheroids showed remarkable new bone formation in vivo,identical to that of the noncryopreserved spheroids even without osteogenic induction. The cryopreserved spontaneous CB-MSC spheroids retained stemness and osteogenic differentiation capability and highlight the utility of spontaneous CB-MSC spheroids as ready-to-use tissue-engineered products for bone tissue engineering.","subitem_description_type":"Abstract"}]},"item_10006_dissertation_number_22":{"attribute_name":"学位授与番号","attribute_value_mlt":[{"subitem_dissertationnumber":"甲第239号"}]},"item_10006_relation_14":{"attribute_name":"情報源","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"雑誌掲載論文(電子版）"}]}]},"item_10006_relation_15":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"Mary Ann Liebert, Inc., publishers"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1089/ten.tec.2021.0001","subitem_relation_type_select":"DOI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"PMC"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8064946/","subitem_relation_type_select":"URI"}}]},"item_10006_relation_23":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"33267737","subitem_relation_type_select":"PMID"}}]},"item_10006_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"©️ Hongwei Dong et al.2021; Published by Mary Ann Liebert, Inc. This Open Access article is distributed under the terms of the Creative Commons License [CC-BY] (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and re- production in any medium, provided the original work is properly cited."}]},"item_10006_source_id_27":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1937-3384","subitem_source_identifier_type":"ISSN"}]},"item_10006_version_type_18":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"董, 宏偉"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-01-06"}],"displaytype":"detail","filename":"33602_A000275_shinsa-yoshi.pdf","filesize":[{"value":"2.5 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"内容の要旨および審査の結果の要旨","url":"https://mdu.repo.nii.ac.jp/record/2976/files/33602_A000275_shinsa-yoshi.pdf"},"version_id":"ba39a724-dbd6-4945-a643-f571b464a449"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-01-06"}],"displaytype":"detail","filename":"33602_A000275_honbun.pdf","filesize":[{"value":"1.4 MB"}],"format":"application/pdf","licensetype":"license_6","mimetype":"application/pdf","url":{"label":"本文","url":"https://mdu.repo.nii.ac.jp/record/2976/files/33602_A000275_honbun.pdf"},"version_id":"a7faa442-798d-4539-9b0b-2c15db0d7c1d"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Cryopreservation","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"spontaneous spheroid","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"osteogenic differentiation capability","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"compact bone-derived mesenchymal stromal cells,","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"MSCs","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"Cryopreserved Spontaneous Spheroids from Compact Bone-Derived Mesenchymal Stromal Cells for Bone Tissue Engineering","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Cryopreserved Spontaneous Spheroids from Compact Bone-Derived Mesenchymal Stromal Cells for Bone Tissue Engineering","subitem_title_language":"en"}]},"item_type_id":"10006","owner":"3","path":["542"],"pubdate":{"attribute_name":"公開日","attribute_value":"2022-01-06"},"publish_date":"2022-01-06","publish_status":"0","recid":"2976","relation_version_is_last":true,"title":["Cryopreserved Spontaneous Spheroids from Compact Bone-Derived Mesenchymal Stromal Cells for Bone Tissue Engineering"],"weko_creator_id":"3","weko_shared_id":3},"updated":"2023-06-19T07:50:51.808001+00:00"}