{"created":"2023-06-19T07:37:19.079215+00:00","id":341,"links":{},"metadata":{"_buckets":{"deposit":"0ce21c33-5829-4ccf-b347-b51ac58d2ee1"},"_deposit":{"created_by":3,"id":"341","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"341"},"status":"published"},"_oai":{"id":"oai:mdu.repo.nii.ac.jp:00000341","sets":["1:13:62"]},"author_link":["0"],"item_2_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1982-12-31","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"230","bibliographicPageStart":"221","bibliographicVolumeNumber":"8","bibliographic_titles":[{"bibliographic_title":"松本歯学"}]}]},"item_2_description_19":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_2_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"From a culture supernatant of P. acnes isolated from a lesion of periodontal disease, hyaluronidase was purified to homogeneity by the sequential procedures including ammonium sulfate precipitation, carboxy methy-cellulose column chromatography, and Sephadex G-100 gel filtration. Specific activity increased 1,027 fold and the recovery of the enzymatic activity was 12.4%. Molecular weight was determined to be 67,000 and isoelectric point was 7.2. Optimal pH for the activity was found at 5.5. The enzyme was inactivated by heating at 60℃ for 10 min. The purified hyaluronidase degraded hyaluronic acid, chondroitin, chondroitin sulfate A and chondroitin sulfate C. From the degradation products of these substrates, unsaturated disaccharides were detected by paper chromatography. When the rate of reaction of this enzyme against hyaluronic acid is supposed to be 100%, the corresponding values against chondroitin, chondroitin sulfate A, and C were 47%, 8%, and 8%, respectively. No degradation by this enzyme of heparin and heparan sulfate was demonstrated.","subitem_description_type":"Abstract"}]},"item_2_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"松本歯科大学学会"}]},"item_2_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00232590","subitem_source_identifier_type":"NCID"}]},"item_2_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0385-1613","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"NAKAMURA, TAKESHI"},{"creatorName":"TANIGUCHI, HIROO"},{"creatorName":"TAKEUCHI, KAZUNORI"},{"creatorName":"KIUCHI, NAOTO"},{"creatorName":"FUJIMURA, SETSUO"}],"nameIdentifiers":[{"nameIdentifier":"0","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2012-05-23"}],"displaytype":"detail","filename":"matsumoto_shigaku_08-02-05.pdf","filesize":[{"value":"916.0 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"matsumoto_shigaku_08-02-05.pdf","url":"https://mdu.repo.nii.ac.jp/record/341/files/matsumoto_shigaku_08-02-05.pdf"},"version_id":"a500bc3b-f6d5-4719-b2d4-1d5c99136b96"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Purification and Properties of Hyaluronidase (EC 4. 2. 2. 1) from an Oral Strain of Propionibacterium acnes","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Purification and Properties of Hyaluronidase (EC 4. 2. 2. 1) from an Oral Strain of Propionibacterium acnes","subitem_title_language":"en"}]},"item_type_id":"2","owner":"3","path":["62"],"pubdate":{"attribute_name":"公開日","attribute_value":"2012-05-23"},"publish_date":"2012-05-23","publish_status":"0","recid":"341","relation_version_is_last":true,"title":["Purification and Properties of Hyaluronidase (EC 4. 2. 2. 1) from an Oral Strain of Propionibacterium acnes"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-06-19T08:40:43.658632+00:00"}