{"created":"2023-06-19T07:37:28.851295+00:00","id":495,"links":{},"metadata":{"_buckets":{"deposit":"af36aa18-8a8a-4365-a2e5-44d3c27b97c2"},"_deposit":{"created_by":3,"id":"495","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"495"},"status":"published"},"_oai":{"id":"oai:mdu.repo.nii.ac.jp:00000495","sets":["1:30:126"]},"author_link":["641"],"item_2_alternative_title_1":{"attribute_name":"その他（別言語等）のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"Structure of catalytic domain of metalloprotease : mainly considering the novel motifidentified on DPP III"}]},"item_2_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2006-04-30","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"20","bibliographicPageStart":"11","bibliographicVolumeNumber":"32","bibliographic_titles":[{"bibliographic_title":"松本歯学"}]}]},"item_2_description_19":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_2_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Almost metalloproteases contain a zinc atom in the active domain coordinating three amino acid residues on the protein and a single water molecule. Metalloproteases are classified into several groups according to the amino acid sequence of the catalytic domain. Dipeptidyl peptidase (DPP) III, which catalyzes the removal of unsubstituted, N-terminal dipeptide from peptides and β-naphthylamide derivatives, had been classified as a serine protease until the amino acid sequence of DPP III was determined. The presence of a similar HELLGH motif on DPP III as the zinc metalloprotease motif HEXXH raises the possibility that DPP III is a metalloprotease. By electrothermal atomic absorption spectrometry, DPP III was demonstrated to contain one mol of zinc per mol of protein, and on zinc-binding study, the dissociation constant (K_d) of DPP III was detected to be (2.5±0.5)×10^<-13> M. These findings show that DPP III is a mono zinc metalloprotease. Furthermore, mutagenesis experiments confirmed that residues His^<450>, His^<455> and Glu^<451> on DPP III are involved in zinc coordination and catalytic activity, and that residue Glu^<508> is a third ligand of zinc atom. DPP III has been newly classified as a metalloprotease containing a novel zinc-binding domain \"HELLGH motif\". The three-dimensional structure of the catalytic domain and putative catalytic mechanism of DPP III are deduced from that of thermolysin proposed by Matthews along with these site-directed mutagenesis studies on DPP III.","subitem_description_type":"Abstract"}]},"item_2_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"松本歯科大学学会"}]},"item_2_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00232590","subitem_source_identifier_type":"NCID"}]},"item_2_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0385-1613","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"深澤, 加與子"}],"nameIdentifiers":[{"nameIdentifier":"641","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2012-05-24"}],"displaytype":"detail","filename":"matsumoto_shigaku_32-01-02.pdf","filesize":[{"value":"2.1 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"matsumoto_shigaku_32-01-02.pdf","url":"https://mdu.repo.nii.ac.jp/record/495/files/matsumoto_shigaku_32-01-02.pdf"},"version_id":"37fe98a3-5178-456b-9ba4-c5eb1be0f32d"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"metalloprotease","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"catalytic domain","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"dipeptidyl peptidase","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"金属プロテアーゼの活性部位の構造 : 新奇な酵素活性部位を持つジペプチジルペプチダーゼⅢを中心に","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"金属プロテアーゼの活性部位の構造 : 新奇な酵素活性部位を持つジペプチジルペプチダーゼⅢを中心に"}]},"item_type_id":"2","owner":"3","path":["126"],"pubdate":{"attribute_name":"公開日","attribute_value":"2012-05-24"},"publish_date":"2012-05-24","publish_status":"0","recid":"495","relation_version_is_last":true,"title":["金属プロテアーゼの活性部位の構造 : 新奇な酵素活性部位を持つジペプチジルペプチダーゼⅢを中心に"],"weko_creator_id":"3","weko_shared_id":3},"updated":"2023-06-19T08:08:44.710049+00:00"}