@article{oai:mdu.repo.nii.ac.jp:00000519,
 author = {深澤, 加與子 and 佐原, 紀行 and 森山, 敬太 and 久野, 知子 and 藤井, 慈貴 and 音琴, 淳一 and 太田, 紀雄 and 宇田川, 信之 and 矢ケ﨑, 裕 and 小澤, 英浩},
 issue = {2},
 journal = {松本歯学},
 month = {Aug},
 note = {application/pdf, Cementum plays an important role in tooth regeneration; however, the organization system has not yet been clarified. We have studied odontoclastic resorption in human deciduous teeth, and found that the cementum completely covers the enamel tissues of horse molar teeth. In order to study the regeneration system ofcementum, an EDTA soluble fraction extracted from horse cementum was analyzed. The 30 kDa protein was isolated from the EDTA fraction of horse cementum by hydroxyapatite chromatography and SDS-PAGE. Tryptic peptides obtained by Edman degradation of the 30 kDa protein were QDTGPRGPRGPAGP(1), GXRGFSLDGVK(2) and GYEFVITEHXLA(3). A search of the UniProt protein sequence database with peptide 1 and peptide 2 showed significant similarity to the two regions of Procollagen α 1 chain, residues 115-128(93%) and 266-277 (82%), respectively. The peptide 3 showed the highest similarity (85%) to the heptapeptide region of mouse Protocadherin 18. The 30 kDa protein would be a product from Type I collagen, a different splicing product translated from a 1 procollagen gene or a novel protein. Protocadherin 18 has not been identified in the oral tissues. This is the first report to find a protocadherin 18-like protein in cementum. Protocadherins are Ca<2+>-dependent adhesion molecules, and are highly expressed in the central nervous system. It is well known that protocadherins are involved in many biological processes such as tissue morphogenesis or cell differentiation events. Therefore, the protocadherin 18-like protein found in cementum might be expected to be involved in the regenerating system of cementum.},
 pages = {160--166},
 title = {ウマ臼歯セメント質中のプロトカドヘリン18様物質の同定},
 volume = {31},
 year = {2005}
}