@article{oai:mdu.repo.nii.ac.jp:00000656,
 author = {ASANUMA, NAOKAZU and ANDO, HIROSHI},
 issue = {1},
 journal = {松本歯学},
 month = {Apr},
 note = {application/pdf, In the apical regions of rabbit taste bud cells, especially on the surface of the microvilli, there exists an enzymatic activity that hydrolyzes an ATP analogue, 5'-adenylylimi-dodiphosphate. Since our previous study had suggested that this activity was that of either ATP pyrophosphatase or adenylyl cyclase, the effects of inhibitors and activators of these enzymes on the enzymatic activity were examined cytochemically. ATP pyrophosphatase inhibitors (dithiothreitol, sodium fluoride and amiloride) reduced the enzymatic activity, while adenylyl cyclase inhibitors (p-chloromercuriphenylsulfonic acid and 5,5'-dithio-bis (2-nitrobenzoic acid)) did not. The effect of a mild activator of ATP pyrophosphatase (sodium azide) was not generally clear. Forskolin, a potent activator of adenylyl cyclase, did not show any enhancing effect. Ca^<2+> enhanced the enzymatic activity. The results indicate that the enzymatic activity is that ofATP pyrophosphatase and probably that of Ca^<2+>-dependent type.},
 pages = {10--20},
 title = {Cytochemical Examination of the AMP-PNP-Hydrolyzing Enzymatic Activity in Rabbit Taste Bud Cells : Effects of Inhibitors and Activators of ATP Pyrophosphatase and of Adenylyl Cyclase},
 volume = {27},
 year = {2001}
}