{"created":"2023-06-19T07:37:38.724443+00:00","id":656,"links":{},"metadata":{"_buckets":{"deposit":"a0b0f809-3c59-4630-aadd-cdcb2496c7a9"},"_deposit":{"created_by":3,"id":"656","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"656"},"status":"published"},"_oai":{"id":"oai:mdu.repo.nii.ac.jp:00000656","sets":["1:35:111"]},"author_link":["0"],"item_2_alternative_title_1":{"attribute_name":"その他（別言語等）のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"ウサギ味蕾細胞におけるAMP-PNP加水分解酵素の細胞化学的研究 : ATPピロホスファターゼおよびアデニル酸シクラーゼの抑制物質・賦活物質の効果"}]},"item_2_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2001-04-30","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"20","bibliographicPageStart":"10","bibliographicVolumeNumber":"27","bibliographic_titles":[{"bibliographic_title":"松本歯学"}]}]},"item_2_description_19":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_2_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"In the apical regions of rabbit taste bud cells, especially on the surface of the microvilli, there exists an enzymatic activity that hydrolyzes an ATP analogue, 5'-adenylylimi-dodiphosphate. Since our previous study had suggested that this activity was that of either ATP pyrophosphatase or adenylyl cyclase, the effects of inhibitors and activators of these enzymes on the enzymatic activity were examined cytochemically. ATP pyrophosphatase inhibitors (dithiothreitol, sodium fluoride and amiloride) reduced the enzymatic activity, while adenylyl cyclase inhibitors (p-chloromercuriphenylsulfonic acid and 5,5'-dithio-bis (2-nitrobenzoic acid)) did not. The effect of a mild activator of ATP pyrophosphatase (sodium azide) was not generally clear. Forskolin, a potent activator of adenylyl cyclase, did not show any enhancing effect. Ca^<2+> enhanced the enzymatic activity. The results indicate that the enzymatic activity is that ofATP pyrophosphatase and probably that of Ca^<2+>-dependent type.","subitem_description_type":"Abstract"}]},"item_2_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"松本歯科大学学会"}]},"item_2_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00232590","subitem_source_identifier_type":"NCID"}]},"item_2_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0385-1613","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"ASANUMA, NAOKAZU"},{"creatorName":"ANDO, HIROSHI"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2012-05-24"}],"displaytype":"detail","filename":"matsumoto_shigaku_27-01-02.pdf","filesize":[{"value":"2.3 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"matsumoto_shigaku_27-01-02.pdf","url":"https://mdu.repo.nii.ac.jp/record/656/files/matsumoto_shigaku_27-01-02.pdf"},"version_id":"a724a83d-e263-4cbb-a14e-dc3d98e9efdc"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"taste bud","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"5'-adenylylimidodiphosphate","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"ATP pyrophosphatase","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"rabbit","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"cytochemistry","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Cytochemical Examination of the AMP-PNP-Hydrolyzing Enzymatic Activity in Rabbit Taste Bud Cells : Effects of Inhibitors and Activators of ATP Pyrophosphatase and of Adenylyl Cyclase","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Cytochemical Examination of the AMP-PNP-Hydrolyzing Enzymatic Activity in Rabbit Taste Bud Cells : Effects of Inhibitors and Activators of ATP Pyrophosphatase and of Adenylyl Cyclase","subitem_title_language":"en"}]},"item_type_id":"2","owner":"3","path":["111"],"pubdate":{"attribute_name":"公開日","attribute_value":"2012-05-24"},"publish_date":"2012-05-24","publish_status":"0","recid":"656","relation_version_is_last":true,"title":["Cytochemical Examination of the AMP-PNP-Hydrolyzing Enzymatic Activity in Rabbit Taste Bud Cells : Effects of Inhibitors and Activators of ATP Pyrophosphatase and of Adenylyl Cyclase"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-06-19T08:33:15.670538+00:00"}