{"created":"2023-06-19T07:37:44.456341+00:00","id":741,"links":{},"metadata":{"_buckets":{"deposit":"e2da7fc5-fbc4-4e42-84ba-de134dc0008e"},"_deposit":{"created_by":3,"id":"741","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"741"},"status":"published"},"_oai":{"id":"oai:mdu.repo.nii.ac.jp:00000741","sets":["1:38:105"]},"author_link":["0"],"item_2_alternative_title_1":{"attribute_name":"その他（別言語等）のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"Porphyromonas gingivalis培養上清中のアルギニン特異的プロテアーゼのアイソザイムの比較"}]},"item_2_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1998-04-30","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"57","bibliographicPageStart":"52","bibliographicVolumeNumber":"24","bibliographic_titles":[{"bibliographic_title":"松本歯学"}]}]},"item_2_description_19":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_2_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"By anion-exchange chromatography of the concentrated culture fluids of P. gingivalis W 50, two proteolytic enzymes, referred to as RGP-I and RGP-II were, eluted from column at the different NaCl concentrations in the eluant buffer. The two enzymes were purified until the preparations displayed single bands with sodium dodecyl sulfate-polyacryl amide gel electrophoresis (SDS-PAGE) by gel filtration and isoelectric focusing. Comparative studies between the two enzymes revealed that the same molecular masses (44 kDa) of RGP-I and II were obtained both with gel filtration and SDS-PAGE. However, the isoelectric points of RGP-I and II were 5.0 and 5.7, respectively. Both enzymes were thiol proteinases and were inhibited by sulfhydryl group blocking reagents, tosyl-L-lysine chloromethylketone, EDTA, leupeptin, L-trans-epoxy-succinylleucylamido-(4-guanidino) butane, and antipain. Both enzymes were activated by glycylglycine. RGP-I and RGP-II hydrolyzed synthetic substrates containing arginine in the P-1 positions, but those containing lysine in the same position were notcleaved.","subitem_description_type":"Abstract"}]},"item_2_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"松本歯科大学学会"}]},"item_2_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00232590","subitem_source_identifier_type":"NCID"}]},"item_2_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0385-1613","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"FUJIMURA, SETSUO"},{"creatorName":"NAKAMURA, TAKESHI"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2012-05-24"}],"displaytype":"detail","filename":"matsumoto_shigaku_24-01-05.pdf","filesize":[{"value":"300.8 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"matsumoto_shigaku_24-01-05.pdf","url":"https://mdu.repo.nii.ac.jp/record/741/files/matsumoto_shigaku_24-01-05.pdf"},"version_id":"52d2ac7f-5572-43d6-bab5-d28497b8699e"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"P. gingivalis","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"proteinases","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"enzymology","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Comparisons of the Isozymes of Arginine-Specific Proteinases in the Culture Supernatant of Porphyromonas gingivalis","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Comparisons of the Isozymes of Arginine-Specific Proteinases in the Culture Supernatant of Porphyromonas gingivalis","subitem_title_language":"en"}]},"item_type_id":"2","owner":"3","path":["105"],"pubdate":{"attribute_name":"公開日","attribute_value":"2012-05-24"},"publish_date":"2012-05-24","publish_status":"0","recid":"741","relation_version_is_last":true,"title":["Comparisons of the Isozymes of Arginine-Specific Proteinases in the Culture Supernatant of Porphyromonas gingivalis"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-06-19T08:31:04.113405+00:00"}