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Contribution of the amino acid residues located near the active site metal to the metal–specific activity of Porphyromonas gingivalis SOD induced by a double mutation of Leu 72 Trp and Leu 76 Phe
https://mdu.repo.nii.ac.jp/records/2269
https://mdu.repo.nii.ac.jp/records/2269fee69821-a3cf-4365-92e4-b32e0d0e60e5
名前 / ファイル | ライセンス | アクション |
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matsumoto_shigaku40-1-04.pdf (1.7 MB)
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Item type | 松本歯学/学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2014-09-11 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Contribution of the amino acid residues located near the active site metal to the metal–specific activity of Porphyromonas gingivalis SOD induced by a double mutation of Leu 72 Trp and Leu 76 Phe | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | site–directed mutagenesis | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | superoxide dismutase | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Porphyromonas gingivalis | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
その他(別言語等)のタイトル | ||||||
その他のタイトル | Porphyromonas gingivalis SOD における活性中心近傍に局在するアミノ酸残基の金属特異的活性における関与:Leu ₇2 Trp およびLeu ₇6 Phe の2残基変異による検討 | |||||
著者 |
MIHARA, MASASHI
× MIHARA, MASASHI× KOMACHIYA, MIHO× MIZOUE, SHINYA× OSAWA, MASAKI× UEMATSU, SETSUKO× KIKUCHI, YUICHIRO× OKUBO, YUICHIRO× HIRAI, KANAME× KUROIWA, AKIHIRO× YAMADA, KAZUHIRO× YAMAKURA, FUMIYUKI× HIRAOKA, B.YUKIHIRO |
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著者別名 |
三原, 正志
× 三原, 正志× 小町谷, 美帆× 溝上, 真也× 大澤, 雅樹× 上松, 節子× 菊池, 有一郎× 大久保, 裕一郎× 平井, 要× 黒岩, 昭弘× 山田, 一尋× 山倉, 文幸× 平岡, 行博 |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | The role of superoxide dismutase (SOD) as a radical scavenger in Porphyromonas gingivalis is well documented. P. gingivalis SOD (Pg SOD), which is characterized by a metal–tolerant activity, can use either iron or manganese as a cofactor. Leu ₇2 and Leu ₇6, located near the active–site metal, are characteristic amino acid sequences of Pg SOD proteins, although they are substituted to Trp in the ₇2 position and Phe in the ₇6 position in most iron–containing SOD (Fe–SOD) proteins. In the present study, we constructed a mutant of the enzyme with changes from Leu ₇2 to Trp and Leu ₇6 to Phe. This mutant SOD was examined with respect to its metal–dependent activity and structural characterization. We herein conclude the integrity of Leu ₇2 and Leu ₇6 is a necessary requisite for the metal–tolerant activity of Pg SOD. | |||||
書誌情報 |
松本歯学 en : Journal of the Matsumoto Dental University Society 巻 40, 号 1, p. 26-34, 発行日 2014-06-30 |
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出版者 | ||||||
出版者 | 松本歯科大学学会 | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0385-1613 | |||||
フォーマット | ||||||
内容記述タイプ | Other | |||||
内容記述 | application/pdf | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 |