WEKO3
アイテム
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Furthermore, mutagenesis experiments confirmed that residues His^\u003c450\u003e, His^\u003c455\u003e and Glu^\u003c451\u003e on DPP III are involved in zinc coordination and catalytic activity, and that residue Glu^\u003c508\u003e is a third ligand of zinc atom. DPP III has been newly classified as a metalloprotease containing a novel zinc-binding domain \"HELLGH motif\". 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金属プロテアーゼの活性部位の構造 : 新奇な酵素活性部位を持つジペプチジルペプチダーゼⅢを中心に
https://mdu.repo.nii.ac.jp/records/495
https://mdu.repo.nii.ac.jp/records/49566a394af-0a22-480b-b386-d43a7a0db6bc
名前 / ファイル | ライセンス | アクション |
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matsumoto_shigaku_32-01-02.pdf (2.1 MB)
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Item type | Journal Article(1) | |||||
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公開日 | 2012-05-24 | |||||
タイトル | ||||||
タイトル | 金属プロテアーゼの活性部位の構造 : 新奇な酵素活性部位を持つジペプチジルペプチダーゼⅢを中心に | |||||
言語 | ||||||
言語 | jpn | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | metalloprotease | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | catalytic domain | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | dipeptidyl peptidase | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
その他(別言語等)のタイトル | ||||||
その他のタイトル | Structure of catalytic domain of metalloprotease : mainly considering the novel motifidentified on DPP III | |||||
著者 |
深澤, 加與子
× 深澤, 加與子 |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Almost metalloproteases contain a zinc atom in the active domain coordinating three amino acid residues on the protein and a single water molecule. Metalloproteases are classified into several groups according to the amino acid sequence of the catalytic domain. Dipeptidyl peptidase (DPP) III, which catalyzes the removal of unsubstituted, N-terminal dipeptide from peptides and β-naphthylamide derivatives, had been classified as a serine protease until the amino acid sequence of DPP III was determined. The presence of a similar HELLGH motif on DPP III as the zinc metalloprotease motif HEXXH raises the possibility that DPP III is a metalloprotease. By electrothermal atomic absorption spectrometry, DPP III was demonstrated to contain one mol of zinc per mol of protein, and on zinc-binding study, the dissociation constant (K_d) of DPP III was detected to be (2.5±0.5)×10^<-13> M. These findings show that DPP III is a mono zinc metalloprotease. Furthermore, mutagenesis experiments confirmed that residues His^<450>, His^<455> and Glu^<451> on DPP III are involved in zinc coordination and catalytic activity, and that residue Glu^<508> is a third ligand of zinc atom. DPP III has been newly classified as a metalloprotease containing a novel zinc-binding domain "HELLGH motif". The three-dimensional structure of the catalytic domain and putative catalytic mechanism of DPP III are deduced from that of thermolysin proposed by Matthews along with these site-directed mutagenesis studies on DPP III. | |||||
書誌情報 |
松本歯学 巻 32, 号 1, p. 11-20, 発行日 2006-04-30 |
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出版者 | ||||||
出版者 | 松本歯科大学学会 | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0385-1613 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AN00232590 | |||||
フォーマット | ||||||
内容記述タイプ | Other | |||||
内容記述 | application/pdf | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 |