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Streptococcus milleriのヒアルロニダーゼの精製とその性状
https://mdu.repo.nii.ac.jp/records/1199
https://mdu.repo.nii.ac.jp/records/11994ce14e63-78d9-4159-9928-8e63708599c6
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
matsumoto_shigaku_14-02-05.pdf (1.2 MB)
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| Item type | Journal Article(1) | |||||||
|---|---|---|---|---|---|---|---|---|
| 公開日 | 2012-05-24 | |||||||
| タイトル | ||||||||
| タイトル | Streptococcus milleriのヒアルロニダーゼの精製とその性状 | |||||||
| 言語 | ||||||||
| 言語 | jpn | |||||||
| キーワード | ||||||||
| 主題Scheme | Other | |||||||
| 主題 | ヒアルロニダーゼ | |||||||
| キーワード | ||||||||
| 主題Scheme | Other | |||||||
| 主題 | 精製 | |||||||
| キーワード | ||||||||
| 言語 | en | |||||||
| 主題Scheme | Other | |||||||
| 主題 | S.milleri | |||||||
| 資源タイプ | ||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||
| 資源タイプ | journal article | |||||||
| その他（別言語等）のタイトル | ||||||||
| その他のタイトル | Purification and Characterization of Hyaluronidase from Streptococcus milleri | |||||||
| 著者 |
吉村, 隆二
× 吉村, 隆二
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| 抄録 | ||||||||
| 内容記述タイプ | Abstract | |||||||
| 内容記述 | Screening tests of gingival crevice dental plaque conducted to detect the bacterial strains responsible for degradation of acid-mucopolysaccharides provided an adequate strain for the production of hyaluronidase. This strain was identified as Streptococcus milleri, based on its various biological properties. The enzyme produced in the culture supernatant of anaerobic culture was purified to homogeneity by sequential procedures including ammonium sulfate precipitation, ionexchange chromatography, and gel filtration. The specific activity increased 15,000 fold and the recovery of the enzyme activity was 21.3%. The molecular weight was 100,000 and its isoelectric point was 9.3. The optimum pH for the activity was 6.0. Heating of the enzyme at 60℃ for 5 minutes resulted in complete inactivation. The enzyme activity was inhibited strongly by Zn^<2+>, Hg^<2+>, and Cu^<2+>, while Ca^<2+>, Mg^<2+>, Fe^<2+>, Co^<2+>, Mn^<2+>, and EDTA had no effect. The enzyme was active against hyaluronic acid, however, chondroitin sulfate, chondroitin sulfate A, B, and C were not degraded by this enzyme. In the enzymatic products of hyaluronic acid, unsaturated disaccharides were detected by the methods of gel filtration and paper chromatography. | |||||||
| 書誌情報 |
松本歯学 巻 14, 号 2, p. 176-184, 発行日 1988-08-31 |
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| 出版者 | ||||||||
| 出版者 | 松本歯科大学学会 | |||||||
| ISSN | ||||||||
| 収録物識別子タイプ | ISSN | |||||||
| 収録物識別子 | 0385-1613 | |||||||
| 書誌レコードID | ||||||||
| 収録物識別子タイプ | NCID | |||||||
| 収録物識別子 | AN00232590 | |||||||
| フォーマット | ||||||||
| 内容記述タイプ | Other | |||||||
| 内容記述 | application/pdf | |||||||
| 著者版フラグ | ||||||||
| 出版タイプ | VoR | |||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||
