| Item type |
松本歯学/学術雑誌論文 / Journal Article(1) |
| 公開日 |
2015-09-18 |
| タイトル |
|
|
タイトル |
Supplementary studies on an extracellular proteinase of Prevotella intermedia: formation and some enzymatic roperties |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
proteinase–Prevotella intermedia–enzyme |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| その他（別言語等）のタイトル |
|
|
その他のタイトル |
Prevotella intermedia の細胞外タンパク分解酵素の補充的研究：産生と酵素性状 |
| 著者 |
KISO, AKIKO
MATSUO, KAZUKO
SHIBATA, YUKINAGA
HASEGAWA, HIROMASA
YOSHIDA, AKIHIRO
FUJIMURA, SETSUO
|
| 著者別名 |
木曽, 有紀子
松尾, 和子
柴田, 幸永
長谷川, 博雅
吉田, 明弘
藤村, 節夫
|
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Formation and some enzymatic properties of a proteinase of Prevotella intermedia ATCC 2₅611 were examined using Azocoll, a solid chromogenic non–specific proteinase substrate. Anaerobic globe box supported better growth of cell and production of proteinase than AnaeroPak. Nearly all proteinase activity was found in the extracellular culture fluid, but not in the cell extract and the envelope. Production of proteinase started immediately after inoculation of the bacteria to the medium and continued until the middle stage of the stationary phase of the growth. Proteinase production was strongly inhibited by addition of glucose or fructose to the medium, moderately by sucrose and weakly by galactose. This proteinase was inactivated entirely by heating at 60℃ for 20 min. Ethyleneglycol bis (β –aminoethyl ether)–N,N,N,N ʼ–tetraacetate (EGTA), Zn2+ and Cu2+ completely inhibited the proteolysis. No effect on the activity was observed by Antipain, SDS, N–tosyl–L–phenylalanyl oromethyl ketone (TPCK), Ca2+ and Mg2+. |
| 書誌情報 |
松本歯学
en : Journal of the Matsumoto Dental University Society
巻 41,
号 1,
p. 1-6,
発行日 2015-06-30
|
| 出版者 |
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|
出版者 |
松本歯科大学学会 |
| ISSN |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
2188-7233 |
| 書誌レコードID |
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|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA12684554 |
| フォーマット |
|
|
内容記述タイプ |
Other |
|
内容記述 |
application/pdf |
| 著者版フラグ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
本文 (965.9 kB)
