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  1. 松本歯学 : Print edition: ISSN 0385-1613 Online edition: ISSN 2188-7233
  2. 41巻(2015)
  3. 1号

Supplementary studies on an extracellular proteinase of Prevotella intermedia: formation and some enzymatic roperties

https://mdu.repo.nii.ac.jp/records/2423
https://mdu.repo.nii.ac.jp/records/2423
13fb4312-9dd3-4c41-835a-7e0f37e1978d
名前 / ファイル ライセンス アクション
matsumoto_shigaku41-1-01.pdf 本文 (965.9 kB)
Item type 学術雑誌論文 / Journal Article(1)
公開日 2015-09-18
タイトル
言語 en
タイトル Supplementary studies on an extracellular proteinase of Prevotella intermedia: formation and some enzymatic roperties
言語
言語 eng
キーワード
言語 en
主題Scheme Other
主題 proteinase–Prevotella intermedia–enzyme
資源タイプ
資源タイプ識別子 http://purl.org/coar/resource_type/c_6501
資源タイプ journal article
その他(別言語等)のタイトル
その他のタイトル Prevotella intermedia の細胞外タンパク分解酵素の補充的研究:産生と酵素性状
著者 KISO, AKIKO

× KISO, AKIKO

WEKO 2783

KISO, AKIKO

Search repository
MATSUO, KAZUKO

× MATSUO, KAZUKO

WEKO 2784

MATSUO, KAZUKO

Search repository
SHIBATA, YUKINAGA

× SHIBATA, YUKINAGA

WEKO 2785

SHIBATA, YUKINAGA

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HASEGAWA, HIROMASA

× HASEGAWA, HIROMASA

WEKO 2786

HASEGAWA, HIROMASA

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YOSHIDA, AKIHIRO

× YOSHIDA, AKIHIRO

WEKO 2787

YOSHIDA, AKIHIRO

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FUJIMURA, SETSUO

× FUJIMURA, SETSUO

WEKO 2788

FUJIMURA, SETSUO

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著者別名 木曽, 有紀子

× 木曽, 有紀子

WEKO 2789

木曽, 有紀子

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松尾, 和子

× 松尾, 和子

WEKO 2790

松尾, 和子

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柴田, 幸永

× 柴田, 幸永

WEKO 2791

柴田, 幸永

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長谷川, 博雅

× 長谷川, 博雅

WEKO 2792

長谷川, 博雅

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吉田, 明弘

× 吉田, 明弘

WEKO 2793

吉田, 明弘

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藤村, 節夫

× 藤村, 節夫

WEKO 2794

藤村, 節夫

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抄録
内容記述タイプ Abstract
内容記述 Formation and some enzymatic properties of a proteinase of Prevotella intermedia ATCC 2₅611 were examined using Azocoll, a solid chromogenic non–specific proteinase substrate. Anaerobic globe box supported better growth of cell and production of proteinase than AnaeroPak. Nearly all proteinase activity was found in the extracellular culture fluid, but not in the cell extract and the envelope. Production of proteinase started immediately after inoculation of the bacteria to the medium and continued until the middle stage of the stationary phase of the growth. Proteinase production was strongly inhibited by addition of glucose or fructose to the medium, moderately by sucrose and weakly by galactose. This proteinase was inactivated entirely by heating at 60℃ for 20 min. Ethyleneglycol bis (β –aminoethyl ether)–N,N,N,N ʼ–tetraacetate (EGTA), Zn2+ and Cu2+ completely inhibited the proteolysis. No effect on the activity was observed by Antipain, SDS, N–tosyl–L–phenylalanyl oromethyl ketone (TPCK), Ca2+ and Mg2+.
書誌情報 松本歯学
en : Journal of the Matsumoto Dental University Society

巻 41, 号 1, p. 1-6, 発行日 2015-06-30
出版者
出版者 松本歯科大学学会
ISSN
収録物識別子タイプ ISSN
収録物識別子 2188-7233
書誌レコードID
収録物識別子タイプ NCID
収録物識別子 AA12684554
フォーマット
内容記述タイプ Other
内容記述 application/pdf
著者版フラグ
出版タイプ VoR
出版タイプResource http://purl.org/coar/version/c_970fb48d4fbd8a85
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