| Item type |
松本歯学/学術雑誌論文 / Journal Article(1) |
| 公開日 |
2015-12-01 |
| タイトル |
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|
タイトル |
Enhanced bone-forming activity of side population cells in the periodontal ligament. |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Side population (SP) cells |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Periodontal ligament (PDL) cells |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Osteogenesis |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Bone regeneration |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 著者 |
Ninomiya, T
Hiraga, T
Hosoya, A
Ohnuma, K
Ito, Y
Takahashi, M
Ito, S
Asashima, M
Nakamura, H
|
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Regeneration of alveolar bone is critical for the successful treatment of periodontal diseases. The periodontal ligament (PDL) has been widely investigated as a source of cells for the regeneration of periodontal tissues. In the present study where we attempted to develop an effective strategy for alveolar bone regeneration, we examined the osteogenic potential of side population (SP) cells, a stem cell-containing population that has been shown to be highly abundant in several kinds of tissues, in PDL cells. Isolated SP cells from the rat PDL exhibited a superior ability to differentiate into osteoblastic cells compared with non-SP (NSP) and unsorted PDL cells in vitro. The mRNA expressions of osteoblast markers and bone morphogenetic protein (BMP) 2 were significantly upregulated in SP cells and were further increased by osteogenic induction. To examine the boneforming activity of SP cells in vivo, PDL SP cells isolated from green fluorescent protein (GFP)-transgenic rats were transplanted with hydroxyapatite (HA) disks into wild-type animals. SP cells exhibited a high ability to induce the mineralized matrix compared with NSP and unsorted PDL cells. At 12 weeks after the implantation, some of the pores in the HA disks with SP cells were filled with mineralized matrices, which were positive for bone matrix proteins, such as osteopontin, bone sialoprotein, and osteocalcin. Furthermore, osteoblast- and osteocyte-like cells on and in the bone-like mineralized matrices were GFP positive, suggesting that the matrices were directly formed by the transplanted cells. These results suggest that PDL SP cells possess enhanced osteogenic potential and could be a potential source for cell-based regenerative therapy for alveolar bone. |
| 書誌情報 |
en : Cell Transplantation
巻 23,
号 6,
p. 691-701,
発行日 2014-04
|
| 出版者 |
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|
出版者 |
Cognizant Communication Corporation |
| ISSN |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
1555-3892 |
| 書誌レコードID |
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|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA1084767X |
| PubMed番号 |
|
|
関連タイプ |
isIdenticalTo |
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|
識別子タイプ |
PMID |
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|
関連識別子 |
info:pmid/23394738 |
| DOI |
|
|
関連タイプ |
isIdenticalTo |
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|
識別子タイプ |
DOI |
|
|
関連識別子 |
https://doi.org/10.3727/096368913X663587 |
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|
関連名称 |
info:doi/10.3727/096368913X663587 |
| 権利 |
|
|
権利情報 |
Copyright © 2014 Ninomiya T et al. Cognizant Comm. Corp. |
| 情報源 |
|
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|
関連名称 |
雑誌掲載論文(電子版) |
| 関連サイト |
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|
識別子タイプ |
URI |
|
|
関連識別子 |
http://www.ingentaconnect.com/content/cog/ct/2014/00000023/00000006/art00001 |
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|
関連名称 |
ingentaconnect |
| フォーマット |
|
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内容記述タイプ |
Other |
|
内容記述 |
application/pdf |
| 著者版フラグ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
2015-ja-001.pdf (651.8 kB)
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