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Salivary histatin 3 inhibits heat shock cognate protein 70–mediated inflammatory cytokine production through toll–like receptors in human gingival fibroblasts.
https://mdu.repo.nii.ac.jp/records/2446
https://mdu.repo.nii.ac.jp/records/244625dc5d29-704a-4972-a30a-ce77ff0738a5
| 名前 / ファイル | ライセンス | アクション |
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2015-ja-006.pdf (1.6 MB)
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| Item type | 松本歯学/学術雑誌論文 / Journal Article(1) | |||||||||
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| 公開日 | 2015-12-01 | |||||||||
| タイトル | ||||||||||
| タイトル | Salivary histatin 3 inhibits heat shock cognate protein 70–mediated inflammatory cytokine production through toll–like receptors in human gingival fibroblasts. | |||||||||
| 言語 | en | |||||||||
| 言語 | ||||||||||
| 言語 | eng | |||||||||
| キーワード | ||||||||||
| 言語 | en | |||||||||
| 主題Scheme | Other | |||||||||
| 主題 | Histatin | |||||||||
| キーワード | ||||||||||
| 言語 | en | |||||||||
| 主題Scheme | Other | |||||||||
| 主題 | Inflammatory cytokine | |||||||||
| キーワード | ||||||||||
| 言語 | en | |||||||||
| 主題Scheme | Other | |||||||||
| 主題 | Toll-like receptor | |||||||||
| キーワード | ||||||||||
| 言語 | en | |||||||||
| 主題Scheme | Other | |||||||||
| 主題 | HSC70 | |||||||||
| 資源タイプ | ||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||
| 資源タイプ | journal article | |||||||||
| 著者 |
Imamura, Y
× Imamura, Y
× Wang, PL
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| 抄録 | ||||||||||
| 内容記述タイプ | Abstract | |||||||||
| 内容記述 | Background: Salivary histatins are bioactive peptides related to the innate immune system associated with antimicrobial activities. However, very little is known about the physiological and biological functions of histatins against host cells or their role in oral cell inflammation. Histatin 3 binds to heat shock cognate protein 70 (HSC70, a constitutively expressed heat shock protein (HSP)). It is unclear whether HSC70 is involved in the inflammatory response in oral cells. Injured oral cells release some intracellular proteins including HSC70. It is possible that released HSC70 induces toll-like receptor (TLR) activation, just as extracellular HSP70 (a stress inducible HSP) does, and that histatin 3 affects this process. Therefore, we tested the hypothesis that HSC70 activates TLR signaling and histatin 3 inhibits this activation and inflammatory cytokine production. Methods: A nuclear factor (NF)-κB-dependent luciferase reporter plasmid was transfected into HEK293 cells stably expressing TLR2 with coreceptor CD14 (293-TLR2/CD14 cells) or stably expressing TLR4 with CD14 and the accessory molecule MD2 (293-TLR4/MD2-CD14 cells). The cells were stimulated with HSC70 in the presence or absence of histatin 3, and examined using luciferase assays. We also stimulated human gingival fibroblasts (HGFs) with HSC70 with or without histatin 3. Then, we analyzed the levels of inflammatory cytokines (interleukin (IL)-6 and IL-8) in the culture media. Cell proteins were analyzed using enzyme-linked immunosorbent assay and Western blotting with antibodies of mitogen-activated protein kinases and NF-κB inhibitor IκB-α, respectively. Histatin 3-bound form of HSC70 was analyzed using limited V8 protease proteolysis. Results: HSC70 induced NF-κB activation in a dose-dependent manner in 293-TLR2/CD14 and 293-TLR4/MD2-CD14 cells, and histatin 3 inhibited this process and when histatin 3 binding to HSC70 was precluded by 15-deoxyspergualin, which augmented NF-κB-triggered activation. In HGFs, histatin 3 also inhibited HSC70-induced inflammatory cytokine production, extracellular signal-regulated protein kinase phosphorylation, and degradation of IκB-α. Moreover, HSC70 in the presence of histatin 3 was relatively resistant to digestion by V8 protease compared with HSC70 in the presence of control peptide. Conclusions: Histatin 3 may be an inhibitor of HSC70-triggered activation of TLR signaling and inflammatory cytokine production and may be involved in inflammation processes noted in oral cells. |
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| 書誌情報 |
en : Journal of inflammation (London, England) 巻 11, p. Article ID:4, 発行日 2014-02-04 |
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| 出版者 | ||||||||||
| 出版者 | BioMed Central Ltd | |||||||||
| ISSN | ||||||||||
| 収録物識別子タイプ | ISSN | |||||||||
| 収録物識別子 | 1476-9255 | |||||||||
| 書誌レコードID | ||||||||||
| 収録物識別子タイプ | NCID | |||||||||
| 収録物識別子 | AA12051004 | |||||||||
| PubMed番号 | ||||||||||
| 関連タイプ | isIdenticalTo | |||||||||
| 識別子タイプ | PMID | |||||||||
| 関連識別子 | info:pmid/24495360 | |||||||||
| DOI | ||||||||||
| 関連タイプ | isIdenticalTo | |||||||||
| 識別子タイプ | DOI | |||||||||
| 関連識別子 | https://doi.org/10.1186/1476-9255-11-4 | |||||||||
| 関連名称 | info:doi/10.1186/1476-9255-11-4 | |||||||||
| 権利 | ||||||||||
| 権利情報 | © 2014 Imamura and Wang; licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. | |||||||||
| 情報源 | ||||||||||
| 関連名称 | 雑誌掲載論文(電子版) | |||||||||
| 関連サイト | ||||||||||
| 識別子タイプ | URI | |||||||||
| 関連識別子 | http://www.journal-inflammation.com/content/11/1/4 | |||||||||
| 関連名称 | BioMed Central | |||||||||
| 関連サイト | ||||||||||
| 識別子タイプ | URI | |||||||||
| 関連識別子 | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3922778/ | |||||||||
| 関連名称 | PubMed Central® (PMC) | |||||||||
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| 内容記述タイプ | Other | |||||||||
| 内容記述 | application/pdf | |||||||||
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| 出版タイプ | VoR | |||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||
