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Histological Evaluation of Periodontal Ligament in Response to Orthodontic Mechanical Stress in Mice
https://mdu.repo.nii.ac.jp/records/2516
https://mdu.repo.nii.ac.jp/records/2516f974fbbd-ded3-4018-83e8-8c34ee7bd7b1
| 名前 / ファイル | ライセンス | アクション |
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本文 (1.2 MB)
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学位論文審査の結果及び最終試験の結果の要旨 (289.9 kB)
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| Item type | 学位論文 / Thesis or Dissertation正(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2016-06-15 | |||||
| タイトル | ||||||
| タイトル | Histological Evaluation of Periodontal Ligament in Response to Orthodontic Mechanical Stress in Mice | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | Periodontal Ligament | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | Orthodontic Mechanical Stress | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | Cell movement | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | Cell differentiation | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | Immunohistochemistry | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_db06 | |||||
| 資源タイプ | doctoral thesis | |||||
| アクセス権 | ||||||
| アクセス権 | open access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
| その他（別言語等）のタイトル | ||||||
| その他のタイトル | 歯科矯正学的メカニカルストレスによるマウス歯周組織改造における細胞動態 | |||||
| 著者 |
Kaneko, Keiko
× Kaneko, Keiko× Matsuda, Sakae× Muraoka, Rina× Nakano, Keisuke× Iwasaki, Takami× Tomida, Mihoko× Tsujigiwa, Hidetsugu× Nagatsuka, Hitoshi× Kawakami, Toshiyuki |
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| 著者別名 |
金子, 圭子
× 金子, 圭子 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | The purpose of the study was to determine the cell dynamics in periodontal ligament in response to mechanical stress during orthodontic movement. Following Waldo’s method, a square sheet of rubber dam was inserted in between the first and second maxillary molars in 10 ddY mice leaving the stress load for 3 hours. After 3 days and at 1 week, cell count on pressure and tension sides of the periodontal ligament was determined. Furthermore, the type of cell present after mechanical stress was identified using GFP bone marrow transplantation mouse model. Immunohistochemistry was carried out at 0 min (immediately after mechanical stress), 24 hours, 1 week, 2 weeks and 6 months. Temporal changes in the expression of GFP-positive bone marrow derived cells were examined. Moreover, double immunofluorescent staining was performed to determine the type of cell in the periodontal ligament. Cell count on the tension side tremendously increased 3 days after mechanical stress. At 1 week, spindle and round cell count increased compared to the control group. These changes were observed on both tension and pressure sides. Cell count on pressure side at 3 days (22.11+/-13.98) and at 1 week (33.23+/-11.39) was higher compared to the control group (15.26+/-8.29). On the tension side, there was a significantly increased at 3 days (35.46+/-11.85), but decreased at 1 week (29.23+/-13.89) although it is still higher compared to the control group (AD+/-SD: 10.37+/-8.69). Using GFP bone marrow transplantation mouse model, GFP positive cell count increased gradually over time in 6 months. GFP positive cells were also positive to CD31, CD68 and Runx2 suggesting that fibroblasts differentiated into osteoclasts and tissue macrophages. In conclusion, mechanical stress during orthodontic movement promoted the increase in the number of cells in the periodontal ligament on both tension and pressure sides. The increase in the number of cells in the periodontal ligament is believed to be due to the migration and cell division of undifferentiated mesenchymal cells. | |||||
| 書誌情報 |
en : International Journal of Medical Sciences 巻 12, 号 9, p. 689-694, 発行日 2015-08-10 |
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| 情報源 | ||||||
| 関連名称 | 雑誌掲載論文(電子版) | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 1449-1907 | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AA1197928X | |||||
| 権利 | ||||||
| 権利情報 | © 2015 Ivyspring International Publisher. This is an open access article distributed under the terms of the Creative Commons Attribution (CC BY-NC) License.(https://creativecommons.org/licenses/by-nc/4.0/) | |||||
| 関連サイト | ||||||
| 識別子タイプ | URI | |||||
| 関連識別子 | http://www.medsci.org/v12p0689.htm | |||||
| 関連名称 | Ivyspring International Publisher | |||||
| 関連サイト | ||||||
| 識別子タイプ | URI | |||||
| 関連識別子 | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4571545/ | |||||
| 関連名称 | PubMed Central® (PMC) | |||||
| PubMed番号 | ||||||
| 識別子タイプ | PMID | |||||
| 関連識別子 | info:pmid/26392805 | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | https://doi.org/10.7150/ijms.12883 | |||||
| 関連名称 | info:doi/10.7150/ijms.12883 | |||||
| フォーマット | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | application/pdf | |||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
| 学位授与年度 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 2015 | |||||
| 報告番号 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 甲第186号 | |||||
| 学位授与番号 | ||||||
| 学位授与番号 | 甲第186号 | |||||
| 学位授与年月日 | ||||||
| 学位授与年月日 | 2016-02-05 | |||||
| 学位名 | ||||||
| 学位名 | 博士（歯学） | |||||
| 学位授与大学 | ||||||
| 学位授与機関識別子Scheme | kakenhi | |||||
| 学位授与機関識別子 | 33602 | |||||
| 学位授与機関名 | 松本歯科大学 | |||||
