| Item type |
学位論文 / Thesis or Dissertation正(1) |
| 公開日 |
2017-05-15 |
| タイトル |
|
|
タイトル |
Migration and Differentiation of GFP-transplanted Bone Marrow-derived Cells into Experimentally Induced Periodontal Polyp in Mice |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Periodontal polyp |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Periodontal ligament |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Cell differentiation |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Immunohistochemistry |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_db06 |
|
資源タイプ |
doctoral thesis |
| アクセス権 |
|
|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| その他（別言語等）のタイトル |
|
|
その他のタイトル |
マウスにおける実験的に発生させた歯根膜ポリープへのGFP移植骨髄由来細胞の移動と分化 |
| 著者 |
MATSUDA, SAEKA
SHOMURA, MASAHITO
OSUGA, NAOTO
TSUJIGIWA, HIDETSUGU
NAKANO, KEISUKE
OKAFUJI, NORIMASA
OCHIAI, TAKANAGA
HASEGAWA, HIROMASA
KAWAKAMI, TOSHIYUKI
|
| 著者別名 |
松田, 紗衣佳
|
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Perforation of floor of the dental pulp is often encountered during root canal treatment in routine clinical practice of dental caries. If perforation were large, granulation tissue would grow to form periodontal polyp. Granulation tissue consists of proliferating cells however their origin is not clear. It was shown that the cells in granulation tissue are mainly from migration of undifferentiated mesenchymal cells of the bone marrow. Hence, this study utilized GFP bone marrow transplantation mouse model. The floor of the pulp chamber in maxillary first molar was perforated using ½ dental round bur. Morphological assessment was carried out by micro CT and microscopy and GFP cell mechanism was further assessed by immunohistochemistry using double fluorescent staining with GFP-S100A4; GFP-Runx2 and GFP-CD31. Results of micro CT revealed alveolar bone resorption and widening of periodontal ligament. Histopathological examination showed proliferation of fibroblasts with some round cells and blood vessels in the granulation tissue. At 2 weeks, the outermost layer of the granulation tissue was lined by squamous cells with distinct intercellular bridges. At 4 weeks, the granulation tissue became larger than the perforation and the outermost layer was lined by relatively typical stratified squamous epithelium. Double immunofluorescent staining of GFP and Runx2 revealed that both proteins were expressed in spindle-shaped cells. Double immunofluorescent staining of GFP and CD31 revealed that both proteins were expressed in vascular endothelial cells in morphologically distinct vessels. The results suggest that fibroblasts, periodontal ligament fibroblasts and blood vessels in granulation tissue were derived from transplanted-bone marrow cells. Thus, essential growth of granulation tissue in periodontal polyp was caused by the migration of undifferentiated mesenchymal cells derived from bone marrow, which differentiated into fibroblasts and later on differentiated into other cells in response to injury. |
| 書誌情報 |
en : International Journal of Medical Sciences
巻 13,
号 7,
p. 500-506,
発行日 2016-06-29
|
| 情報源 |
|
|
|
関連名称 |
雑誌掲載論文(電子版) |
| ISSN |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
1449-1907 |
| 権利 |
|
|
権利情報 |
Copyright: 2016 © Ivyspring International Publisher. This is an open access article distributed under the terms of the Creative Commons Attribution (CC BY-NC) License. See http://ivyspring.com/terms for full terms and conditions. |
| 関連サイト |
|
|
|
識別子タイプ |
URI |
|
|
関連識別子 |
http://www.medsci.org/v13p0500.htm |
|
|
関連名称 |
Ivyspring International Publisher |
| PubMed番号 |
|
|
|
識別子タイプ |
PMID |
|
|
関連識別子 |
info:pmid/27429586 |
| DOI |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
https://doi.org/10.7150/ijms.15671 |
|
|
関連名称 |
info:doi/10.7150/ijms.15671 |
| フォーマット |
|
|
内容記述タイプ |
Other |
|
内容記述 |
application/pdf |
| 著者版フラグ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| 学位授与年度 |
|
|
内容記述タイプ |
Other |
|
内容記述 |
2016 |
| 報告番号 |
|
|
内容記述タイプ |
Other |
|
内容記述 |
甲第202号 |
| 学位授与番号 |
|
|
学位授与番号 |
甲第202号 |
| 学位授与年月日 |
|
|
学位授与年月日 |
2017-02-09 |
| 学位名 |
|
|
学位名 |
博士（歯学） |
| 学位授与大学 |
|
|
|
学位授与機関名 |
松本歯科大学 |
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