| Item type |
学術雑誌論文 / Journal Article_02(1) |
| 公開日 |
2018-11-15 |
| タイトル |
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タイトル |
Osteogenic Factor Runx2 Marks a Subset of Leptin Receptor-Positive Cells that Sit Atop the Bone Marrow Stromal Cell Hierarchy |
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言語 |
en |
| 言語 |
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言語 |
eng |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| 著者 |
Yang, Mengyu
Arai, Atsushi
Udagawa, Nobuyuki
Hiraga, Toru
Lijuan, Zhao
Ito, Susumu
Komori, Toshihisa
Moriishi, Takeshi
Matsuo, Koichi
Shimoda, Kouji
Zahalka, Ali H
Kobayashi, Yasuhiro
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| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
Bone marrow mesenchymal stem and progenitor cells (BM-MSPCs) maintain homeostasis of bone tissue by providing osteoblasts. Although several markers have been identified for labeling of MSPCs, these labeled cells still contain non-BM-MSPC populations. Studies have suggested that MSPCs are observed as leptin receptor (LepR)-positive cells, whereas osteoblasts can be classified as positive for Runx2, a master regulator for osteoblastogenesis. Here, we demonstrate, using Runx2-GFP reporter mice,that the LepR-labeled population contains Runx2-GFPlow sub-population, which possesses higher fibroblastic colony-forming units (CFUs) and mesensphere capacity, criteria for assessing stem cell activity, than the Runx2-GFP− population. In response to parathyroid hormone (PTH), a bone anabolic hormone, LepR+Runx2-GFPlow cells increase Runx2 expression and form multilayered structures near the bone surface. Subsequently, the multilayered cells express Osterix and Type I collagen α, resulting in generation of mature osteoblasts. Therefore, our results indicate that Runx2 is weakly expressed in the LepR+ population without osteoblastic commitment, and the LepR+Runx2-GFPlow stromal cells sit atop the BM stromal hierarchy. |
| 書誌情報 |
en : Scientific reports.
巻 7,
号 1,
p. 4928,
発行日 2017-07-10
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| 出版者 |
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出版者 |
Nature Publishing Group |
| ISSN |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
2045-2322 |
| DOI |
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関連タイプ |
isIdenticalTo |
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識別子タイプ |
DOI |
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関連識別子 |
https://doi.org/10.1038/s41598-017-05401-1 |
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関連名称 |
info:doi/10.1038/s41598-017-05401-1. |
| 権利 |
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権利情報 |
© Mengyu Yang(et al) 2017 This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
| 情報源 |
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関連名称 |
雑誌掲載論文(電子版) |
| 関連サイト |
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識別子タイプ |
URI |
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関連識別子 |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5503992/ |
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関連名称 |
PubMed Central® (PMC) |
| 関連サイト |
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識別子タイプ |
URI |
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関連識別子 |
https://www.nature.com/articles/s41598-017-05401-1 |
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関連名称 |
the publishers of Nature. |
| フォーマット |
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内容記述タイプ |
Other |
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内容記述 |
application/pdf |
| 著者版フラグ |
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出版タイプ |
VoR |
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出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
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