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Characterization of spontaneous spheroids from oral mucosa-derived cells and their direct comparison with spheroids from skin-derived cells
https://mdu.repo.nii.ac.jp/records/2909
https://mdu.repo.nii.ac.jp/records/2909e92fb9d6-4b74-4d5d-8e45-32061049e886
| 名前 / ファイル | ライセンス | アクション |
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内容の要旨および審査の結果の要旨 (758.1 kB)
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| Item type | 学位論文 / Thesis or Dissertation正(1) | |||||||
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| 公開日 | 2020-05-01 | |||||||
| タイトル | ||||||||
| タイトル | Characterization of spontaneous spheroids from oral mucosa-derived cells and their direct comparison with spheroids from skin-derived cells | |||||||
| 言語 | en | |||||||
| 言語 | ||||||||
| 言語 | eng | |||||||
| 資源タイプ | ||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_db06 | |||||||
| 資源タイプ | doctoral thesis | |||||||
| アクセス権 | ||||||||
| アクセス権 | open access | |||||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||||
| その他（別言語等）のタイトル | ||||||||
| その他のタイトル | 口腔粘膜由来細胞から形成された自発的スフェロイドの特性評価と皮膚由来細胞によるスフェロイドとの性質の違い | |||||||
| 著者 |
李, 妮
× 李, 妮
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| 著者別名 |
Li, Ni
× Li, Ni
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| 抄録 | ||||||||
| 内容記述タイプ | Abstract | |||||||
| 内容記述 | Abstract Background: Our group has developed a novel method for spontaneous spheroid formation using a specific lowadherence culture plate with around 90° water contact angle. In this study, this method was applied for oral mucosa-derived cells. First, the feasibility of spontaneous spheroid formation was tested. Next, the characteristics of spontaneous spheroids from oral mucosa- and skin-derived cells were compared with special focus on the stemness and neuronal differentiation capability. Methods: Oral mucosal cells were obtained from the palate and buccal mucosa of C57BL/6J mice. Similarly, skin cells were obtained from the back of the same mouse strain. Passage 2–3 cells were inoculated into the specific low-adherence culture plates to form spontaneous spheroids. The effect of basic fibroblast growth factor (bFGF), epidermal growth factor (EGF), and B27 supplement on spheroid formation and maintenance was assessed. Immunofluorescence and quantitative reverse transcription polymerase chain reaction (qRT-PCR) were performed to investigate the expression of pluripotency markers, cell proliferation and apoptosis markers, and neurogenic differentiation markers. Results: Using this culture plate, spontaneous spheroid formation was feasible. This process depended on the presence of serum but was independent of the additives such as bFGF, EGF, and B27 supplement, although they improved the efficiency and were essential for spheroid maintenance. This result was confirmed by the higher expression of Caspase7 in the spheroids cultured without the additives than that with the additives. The spheroids from oral mucosa-derived cells expressed stem cell markers, such as Sox2, SSEA1, Oct4, Nanog, and Nestin. The expression of Sox2 in spheroids from oral mucosal cells was higher than that in spheroids from skin-derived cells. Both spheroid-forming cell types had the ability to differentiate into neural and Schwann cells after neurogenic induction, although significantly higher MAP 2, MBP, Nestin, and Nurr1 gene expression was noted in the cells from oral mucosa-derived spheroids. Conclusions: The results showed that spontaneous spheroids from oral mucosa-derived cells contain highly potent stem cells, which were as good as skin-derived stem cells. The high expression of certain neuronal marker genes suggests an advantage of these cells for regeneration therapy for neuronal disorders. | |||||||
| 書誌情報 |
en : Stem Cell Research & Thepapy 巻 10, 号 1, p. 184, 発行日 2019 |
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| 情報源 | ||||||||
| 関連名称 | 雑誌掲載論文(電子版） | |||||||
| ISSN | ||||||||
| 収録物識別子タイプ | ISSN | |||||||
| 収録物識別子 | 1757-6512 | |||||||
| 権利 | ||||||||
| 権利情報 | © The Author(s). 2019 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/) | |||||||
| 関連サイト | ||||||||
| 識別子タイプ | URI | |||||||
| 関連識別子 | https://stemcellres.biomedcentral.com/track/pdf/10.1186/s13287-019-1283-0 | |||||||
| 関連名称 | stemcellres.biomedcentral.com | |||||||
| 関連サイト | ||||||||
| 識別子タイプ | URI | |||||||
| 関連識別子 | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6591807/ | |||||||
| 関連名称 | PMC | |||||||
| PubMed番号 | ||||||||
| 識別子タイプ | PMID | |||||||
| 関連識別子 | 31234925 | |||||||
| フォーマット | ||||||||
| 内容記述タイプ | Other | |||||||
| 内容記述 | application/pdf | |||||||
| 著者版フラグ | ||||||||
| 出版タイプ | VoR | |||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||
| 学位授与年度 | ||||||||
| 内容記述タイプ | Other | |||||||
| 内容記述 | 2019 | |||||||
| 報告番号 | ||||||||
| 内容記述タイプ | Other | |||||||
| 内容記述 | 甲第220号 | |||||||
| 学位授与番号 | ||||||||
| 学位授与番号 | 甲第220号 | |||||||
| 学位授与年月日 | ||||||||
| 学位授与年月日 | 2020-02-06 | |||||||
| 学位名 | ||||||||
| 学位名 | 博士(歯学） | |||||||
| 学位授与大学 | ||||||||
| 学位授与機関名 | 松本歯科大学 | |||||||
