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Spontaneously Formed Spheroids from Mouse Compact Bone-Derived Cells Retain Highly Potent Stem Cells with Enhanced Differentiation Capability
https://mdu.repo.nii.ac.jp/records/2910
https://mdu.repo.nii.ac.jp/records/2910a0edd164-763a-4628-8d30-e558f982747c
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
内容の要旨および審査の結果の要旨 (649.2 kB)
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| Item type | 学位論文 / Thesis or Dissertation正(1) | |||||
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| 公開日 | 2020-05-01 | |||||
| タイトル | ||||||
| タイトル | Spontaneously Formed Spheroids from Mouse Compact Bone-Derived Cells Retain Highly Potent Stem Cells with Enhanced Differentiation Capability | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_db06 | |||||
| 資源タイプ | doctoral thesis | |||||
| アクセス権 | ||||||
| アクセス権 | open access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
| その他（別言語等）のタイトル | ||||||
| その他のタイトル | マウス緻密骨由来細胞から形成された自発的スフェロイドは高い幹細胞性と分化能力を持つ | |||||
| 著者 |
陳, 凱
× 陳, 凱 |
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| 著者別名 |
Chin, Kai
× Chin, Kai |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | The results from our recent study showed the presence of two distinct spheroid-forming mechanisms, i.e., spontaneous and mechanical. In this study, we focused on the spontaneously formed spheroids, and the character of spontaneously formed spheroids from mouse compact bone-derived cells (CBDCs) was explored. Cells from (C57BL/6J) mouse leg bones were isolated, and compact bone-derived cells were cultured after enzymatic digestion. Spontaneous spheroid formation was achieved on a culture plate with specific water contact angle as reported. The expression levels of embryonic stem cell markers were analyzed using immunofluorescence and quantitative reverse transcription polymerase chain reaction. Then, the cells from spheroids were induced into osteogenic and neurogenic lineages. The spontaneously formed spheroids from CBDCs were positive for ES cell markers such as SSEA1, Sox2, Oct4, and Nanog. Additionally, the expressions of fucosyltransferase 4/FUT4 (SSEA1), Sox2, and Nanog were significantly higher than those in monolayer cultured cells. The gene expression of mesenchymal stem cell markers was almost identical in both spheroids and monolayer-cultured cells, but the expression of Sca-1 was higher in spheroids. Spheroid-derived cells showed significantly higher osteogenic and neurogenic marker expression than monolayer-cultured cells after induction. Spontaneously formed spheroids expressed stem cell markers and showed enhanced osteogenic and neurogenic differentiation capabilities than cells from the conventional monolayer culture, which supports the superior stemness. | |||||
| 書誌情報 |
en : Stem Cells Internationa 巻 2019, 号 May, p. 5, 発行日 2019 |
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| 情報源 | ||||||
| 関連名称 | 雑誌掲載論文（電子版） | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 1687-9678 | |||||
| 権利 | ||||||
| 権利情報 | Copyright © 2019 Kai Chen et al. This is an open access article distributed under the Creative Commons Attribution License, Attribution 4.0 International (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. | |||||
| 関連サイト | ||||||
| 識別子タイプ | URI | |||||
| 関連識別子 | https://www.hindawi.com/journals/sci/2019/8469012/ | |||||
| 関連名称 | hindawi.com/journals/sci/2019/8469012/ | |||||
| 関連サイト | ||||||
| 識別子タイプ | URI | |||||
| 関連識別子 | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6525826/ | |||||
| 関連名称 | PMC | |||||
| PubMed番号 | ||||||
| 識別子タイプ | PMID | |||||
| 関連識別子 | 31191686 | |||||
| フォーマット | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | application/pdf | |||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
| 学位授与年度 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 2019 | |||||
| 報告番号 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 甲第221号 | |||||
| 学位授与番号 | ||||||
| 学位授与番号 | 甲第221号 | |||||
| 学位授与年月日 | ||||||
| 学位授与年月日 | 2020-02-06 | |||||
| 学位名 | ||||||
| 学位名 | 博士(歯学） | |||||
| 学位授与大学 | ||||||
| 学位授与機関識別子Scheme | kakenhi | |||||
| 学位授与機関識別子 | 33602 | |||||
| 学位授与機関名 | 松本歯科大学 | |||||
