| Item type |
松本歯学/学術雑誌論文 / Journal Article(1) |
| 公開日 |
2021-03-16 |
| タイトル |
|
|
タイトル |
Preference for threonine over serine near the active site metal of superoxide dismutase in Porphyromonas gingivalis:Effect of Gly 155 to Ser mutation |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
site–directed mutagenesis |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
superoxide dismutase |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Porphyromonas gingivalis |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| その他（別言語等）のタイトル |
|
|
その他のタイトル |
Porphyromonas gingivalis SOD の活性中心金属の近位には，トレオニンがセリンよりも優先的に選択される:155位Gly をSer に変異させた影響 |
| 著者 |
MIHARA, MASASHI
KOMACHIYA, MIHO
ARAI, ATSUSHI
KAWAHARA, YOSHIMI
OKUBO, YUICHIRO
YAMAKURA, FUMIYUKI
HIROOKA, YUKIHIRO
|
| 著者別名 |
三原, 正志
小町谷, 美帆
荒井, 敦
川原, 良美
大久保, 裕一郎
山倉, 文幸
平岡, 行博
|
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Summary In this study, we analyzed the cambialistic superoxide dismutases (SODs) of Porphyromonas gingivalis (Pg–SODs) with a mutation directed at glycine position 155 to introduce serine. Glycine 155 is a highly conserved outer sphere in manganese–containing SODs (Mn–SODs), even though threonine is substituted at this position in most iron–containing SODs (Fe–SODs). Conversion of glycine 155 may affect the metal–specific activity of SODs, including that of cambialistic Pg–SODs.Previously, we reported that a Pg–SOD Gly155Thr mutant exhibited a substantially changed metalspecific activity from that of a cambialistic type to an Fe–specific type. Although serine and threonine equally contribute to protein function, serine has never been observed at position 155 in SODs. In order to elucidate this phenomenon, we created a Pg–SOD mutant Gly155Ser. The specific Fedependent activity of this mutant was almost identical to the wild–type SOD, whereas the Mndependent activity exhibited a 60% reduction. The ultraviolet–visible absorption of Fe– and Mnreconstituted mutant SODs did not exhibit characteristic absorption spectra. Similar to the wild–typeSODs, the mutant SODs exhibited a single band with identical mobilities after separation by polyacrylamide gel electrophoresis. However, their behavior after anion–exchange chromatography differed from that of the wild–type SODs. Thus, Gly155 is considered to be an essential residue for maintaining the hydrogen–bond network for Mn–specific and Fe/Mn–tolerant activity. Gly155 was possibly retained instead of Ser during the evolution of SODs owing to its increased efficiency in maintaining a dimeric structure. |
| 書誌情報 |
松本歯学
en : Journal of the Matsumoto Dental University Society
巻 46,
号 2,
p. 59-69,
発行日 2021-03-16
|
| 出版者 |
|
|
出版者 |
松本歯科大学学会 |
| ISSN |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
2188-7233 |
| 書誌レコードID |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA12684554 |
| フォーマット |
|
|
内容記述タイプ |
Other |
|
内容記述 |
application/pdf |
| 著者版フラグ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
本文 (3.1 MB)
