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内容記述 |
ABSTRACT This study compared the differences in salivary and fecal bacterial flora between 80- and 100-year-old people in different independent states to provide a reference for the bacterial composition of the digestive tract in older people of different ages. Saliva and fecal samples were collected from eight centenarians and 10 octogenarians whose Barthel index (BI) is less than 60,which means poor independence, as well as 10 independent octogenarians from nursing homes.A dentist counted the number of teeth, and evaluated the denture and nutritional status. Bacterial DNA was extracted and multiplex 16S rRNA (V3–V4)sequencing was performed using the Illumina MiSeq platform.Composition and diversity were analyzed by LEfSe,student’s t-test, weighted PcoA distance, and Anosim analysis. Older people who were more independent had significantly better nutritional intake than those who were less independent, and this difference was not reflected in age.The digestive tract of well independent seniors has more normal flora,which also reflects the link between general health and gut flora.While the saliva of less independent seniors contains more pathogenic bacteria,which may be a result of their inability to effectively clean their oral hygiene due to their poor functional independence.In addition, Akkermansia, a genus of bacteria commonly considered to be probiotics,was more frequently found in the stools of the less independent eighty-year-olds.There was no significant difference in the intestinal microbiota of 80-year-old and 100-year-old individuals with low functional independence in the β diversity that constitutes the microbiota.On the other hand,there was a significant difference in β diversity in the salivary flora.The reason for this was thought to be the difference in the number of remaining teeth between 80-year-old and 100-year-old subjects.In addition, there was a significant difference in β diversity in both the intestinal and salivary microbiota between the 80-year-old group with low functional independence and the normal 80-year-old group.From the above, it was clarified that functional independence affects the diversity that constitutes the salivary and gut microbiota.In addition, it was suggested that functional independence affects the diversity of salivary and intestinal microbiota more than age. |
内容の要旨および審査の結果の要旨 (1.7 MB)
