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Comparative Studies of Acid and Alkaline Phosphatases from Bacteroides melaninogenicus
https://mdu.repo.nii.ac.jp/records/351
https://mdu.repo.nii.ac.jp/records/35151eb7667-4ed2-4c85-972b-31367e2cf8cb
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
matsumoto_shigaku_09-01-04.pdf (752.1 kB)
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| Item type | Journal Article(1) | |||||||
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| 公開日 | 2012-05-23 | |||||||
| タイトル | ||||||||
| タイトル | Comparative Studies of Acid and Alkaline Phosphatases from Bacteroides melaninogenicus | |||||||
| 言語 | en | |||||||
| 言語 | ||||||||
| 言語 | eng | |||||||
| キーワード | ||||||||
| 言語 | en | |||||||
| 主題Scheme | Other | |||||||
| 主題 | B. melaninogenicus | |||||||
| キーワード | ||||||||
| 言語 | en | |||||||
| 主題Scheme | Other | |||||||
| 主題 | phosphatases | |||||||
| キーワード | ||||||||
| 言語 | en | |||||||
| 主題Scheme | Other | |||||||
| 主題 | purification | |||||||
| キーワード | ||||||||
| 言語 | en | |||||||
| 主題Scheme | Other | |||||||
| 主題 | properties | |||||||
| 資源タイプ | ||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||
| 資源タイプ | journal article | |||||||
| 著者 |
FUJIMURA, SETSUO
× FUJIMURA, SETSUO
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| 抄録 | ||||||||
| 内容記述タイプ | Abstract | |||||||
| 内容記述 | Acid and alkaline phosphatases from an oral strin of Bacteroides melaninogenicus were purified to homogeneity from cell extracts and their properties were compared. Molecular weights of acid and alkaline phosphatases were estimated to be 62,000 and 160,000, respectively. Michaelis constant for p-nitrophenylphosphate of the acid phosphatase was 0.17 mM and that of the alkaline enzyme was 0.23 mM. The alkaline phosphatase was more stable than the acid phospatase when they were heated at 60℃. The activity of the acid phosphatase was largely reduced by Cu^<2+> or fluoride and the alkaline phosphatase was quite sensitive to inhibition by Zn^<2+>, thiol compounds, or EDTA. The inactivation by EDTA of the alkaline phosphatase was restored with Ca^<2+> or Mg^<2+>. The acid phosphatase hydrolyzed α-D-glucose 1.6-diphosphate, p-nitrophenylphos-phate, D-glucose 6-phosphate, and D-fructose 6-phosphate. On the other hand, p-nitro-phenylphosphate was the most suitable substrate for the alkaline phosphatase. Nucleoside triphosphates were also hydrolyzed by the alkaline phosphatase. | |||||||
| 書誌情報 |
松本歯学 巻 9, 号 1, p. 18-27, 発行日 1983-06-30 |
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| 出版者 | ||||||||
| 出版者 | 松本歯科大学学会 | |||||||
| ISSN | ||||||||
| 収録物識別子タイプ | ISSN | |||||||
| 収録物識別子 | 0385-1613 | |||||||
| 書誌レコードID | ||||||||
| 収録物識別子タイプ | NCID | |||||||
| 収録物識別子 | AN00232590 | |||||||
| フォーマット | ||||||||
| 内容記述タイプ | Other | |||||||
| 内容記述 | application/pdf | |||||||
| 著者版フラグ | ||||||||
| 出版タイプ | VoR | |||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||
