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走査電子顕微鏡によるマウス顎下腺の細胞内構造の観察
https://mdu.repo.nii.ac.jp/records/232
https://mdu.repo.nii.ac.jp/records/232c91cc778-aed2-44dc-9fe9-06af6e05c45e
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
matsumoto_shigaku_05-02-08.pdf (2.9 MB)
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| Item type | Journal Article(1) | |||||||
|---|---|---|---|---|---|---|---|---|
| 公開日 | 2012-05-23 | |||||||
| タイトル | ||||||||
| タイトル | 走査電子顕微鏡によるマウス顎下腺の細胞内構造の観察 | |||||||
| 言語 | ||||||||
| 言語 | jpn | |||||||
| 資源タイプ | ||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||
| 資源タイプ | journal article | |||||||
| その他（別言語等）のタイトル | ||||||||
| その他のタイトル | Scanning Electron Microscopic Study of Intracellar Stractures of the Mouse Submandibular Gland | |||||||
| 著者 |
佐原, 紀行
× 佐原, 紀行
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| 抄録 | ||||||||
| 内容記述タイプ | Abstract | |||||||
| 内容記述 | A variety of procedures have been employed to prepare soft tissues and cells to observe their intracellar stractures by the scanning electron microscope. However, their procedures often unsatisfactory because of artifact caused by shearing forces and other reasons. The present study attempt to examine successful procedure for intracellar stractures of mouse submandibular glands. After cryofracture was used, mild ion-etching (700V) or low-pH, hypotonic solution (without ion-etching) method was applicated for disclose intracellar stractures of their cells. Ion-etching specimens were examined by three diffent fixations and effect of ionetching time. Specimens fixed with paraformaldehyde and glutaraldehyde resulted in better ionetching effect than those fixed with osmium tetroxide. Successuful result for disclose intracellar stractures was obtained from paraformaldehyde and glutaraldehyde fixation, and ion-etching specimens for 10-15 minutes. Without ion-etching method did not get good results in submandibular glands in present study. | |||||||
| 書誌情報 |
松本歯学 巻 5, 号 2, p. 181-190, 発行日 1979-12-31 |
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| 出版者 | ||||||||
| 出版者 | 松本歯科大学学会 | |||||||
| ISSN | ||||||||
| 収録物識別子タイプ | ISSN | |||||||
| 収録物識別子 | 0385-1613 | |||||||
| 書誌レコードID | ||||||||
| 収録物識別子タイプ | NCID | |||||||
| 収録物識別子 | AN00232590 | |||||||
| フォーマット | ||||||||
| 内容記述タイプ | Other | |||||||
| 内容記述 | application/pdf | |||||||
| 著者版フラグ | ||||||||
| 出版タイプ | VoR | |||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||
