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Purification and Partial Characterization of Leucine Aminopeptidase from Actinomyces viscosus
https://mdu.repo.nii.ac.jp/records/1381
https://mdu.repo.nii.ac.jp/records/13815715a91c-a873-4444-b2f8-028952009d0b
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
matsumoto_shigaku_10-02-03.pdf (505.6 kB)
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| Item type | Journal Article(1) | |||||||
|---|---|---|---|---|---|---|---|---|
| 公開日 | 2012-05-24 | |||||||
| タイトル | ||||||||
| タイトル | Purification and Partial Characterization of Leucine Aminopeptidase from Actinomyces viscosus | |||||||
| 言語 | en | |||||||
| 言語 | ||||||||
| 言語 | eng | |||||||
| キーワード | ||||||||
| 言語 | en | |||||||
| 主題Scheme | Other | |||||||
| 主題 | Leucine aminopeptidase | |||||||
| キーワード | ||||||||
| 言語 | en | |||||||
| 主題Scheme | Other | |||||||
| 主題 | purification | |||||||
| キーワード | ||||||||
| 言語 | en | |||||||
| 主題Scheme | Other | |||||||
| 主題 | A.viscosus | |||||||
| 資源タイプ | ||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||
| 資源タイプ | journal article | |||||||
| 著者 |
NAKAMURA, TAKESHI
× NAKAMURA, TAKESHI
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| 抄録 | ||||||||
| 内容記述タイプ | Abstract | |||||||
| 内容記述 | Leucine aminopeptidase was purified from cell-free extracts of Actinomyces viscosus ATCC 19246 and some properties were investigated. The enzyme had a molecular weight of 65,000 and its isoelectric point was 4.0. Optimum pH was found at 7.0. The enzyme was quite labile over 40℃. It was sensitive to inhibition by diisopropylfluorophosphate, phenylmethane sulfonylfluoride, or tosyl-L-lysine chloromethyl ketone. Inhibition by urea was also obvious and this inhibition was found to be irreversible. Ca^<2+>, Mg^<2+>, Mn^<2+> or Co^<2+> had no no effect on the activity. Leucine-P-nitroanilide was the most suitable substrate among the tested synthetic substrates. | |||||||
| 書誌情報 |
松本歯学 巻 10, 号 2, p. 130-135, 発行日 1984-12-31 |
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| 出版者 | ||||||||
| 出版者 | 松本歯科大学学会 | |||||||
| ISSN | ||||||||
| 収録物識別子タイプ | ISSN | |||||||
| 収録物識別子 | 0385-1613 | |||||||
| 書誌レコードID | ||||||||
| 収録物識別子タイプ | NCID | |||||||
| 収録物識別子 | AN00232590 | |||||||
| フォーマット | ||||||||
| 内容記述タイプ | Other | |||||||
| 内容記述 | application/pdf | |||||||
| 著者版フラグ | ||||||||
| 出版タイプ | VoR | |||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||
