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Subcutaneous basic FGF-Injection accelerates the development of mandibular condyle of newborn mice during lactation period
https://mdu.repo.nii.ac.jp/records/2229
https://mdu.repo.nii.ac.jp/records/2229b5c19d14-2994-4beb-b3b6-f44cb115545c
| 名前 / ファイル | ライセンス | アクション |
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本文 (5.2 MB)
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内容の要旨および審査の結果の要旨 (315.3 kB)
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| Item type | 学位論文 / Thesis or Dissertation正(1) | |||||
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| 公開日 | 2014-07-15 | |||||
| タイトル | ||||||
| タイトル | Subcutaneous basic FGF-Injection accelerates the development of mandibular condyle of newborn mice during lactation period | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | Basic FGF | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | Condyle development | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | Local administration | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | Newborn mice | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_db06 | |||||
| 資源タイプ | doctoral thesis | |||||
| アクセス権 | ||||||
| アクセス権 | open access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
| その他（別言語等）のタイトル | ||||||
| その他のタイトル | bFGFの皮下注射は授乳期における新生児マウスの下顎頭の発育を促進する | |||||
| 著者 |
Aoyama, Yuki
× Aoyama, Yuki× Ochiai, Takanaga× Shen, FA-Chin× Hasegawa, Hiromasa |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | To elucidate the effect of local bFGF administration, we administered a total of 0.3μg rhbFGF was to the left mandibular condyle by three injections of 0.1μl bFGF solution for 3 days after birth. The contralateral condyles with three injections of 0.1μl physiologic saline served as controls. Serial sections including the widest condyle were evaluated with H-E staining and immunostaining for PCNA. The maximum width of condyle, proliferative zone thickness, proliferative zone thickness ratio and proliferating index were analyzed using image analysis software after digital image capture of H-E stained sections. All condyles were composed of fibrous, proliferative, maturation and hypertrophic cell zones without significant abnormal findings. Experimental condyles demonstrated a markedly thickened proliferative zone compared with that of the controls at 1 day after the injection. At 4 and 7 days, chondrocytes and hypertrophic chondrocytes of the experiments increased in thickness. However, at 14 days after the injection, both the controls and experiments showed similar features with an upward shift of ossification front and active formation of bone trabeculae. Morphometrically, all values of experiments were significantly higher than those of the controls. Especially at 4 days after the final injection, proliferative zone thickness and proliferative index values of the experiment reached a peak and were nearly 1.6-fold higher than those of the control, which were statistically significant compared to other experimental groups. Despite the short half-life of rhbFGF, its effect seems to be prolonged by synergic actions of growth factors such as bFGF or TGF beta1. In conclusion, we showed that local administration of bFGF was feasible for accelerating mesenchymal cell proliferation of mandibular condyles of newborn mice in the lactation period after birth. | |||||
| 書誌情報 |
en : Journal of hard tissue biology 巻 23, 号 3, p. 293-300, 発行日 2012-07 |
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| 情報源 | ||||||
| 関連名称 | 雑誌掲載論文(電子版) | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 1341-7649 | |||||
| 権利 | ||||||
| 権利情報 | © 2013 The Hard Tissue Biology Network Association | |||||
| 関連サイト | ||||||
| 識別子タイプ | URI | |||||
| 関連識別子 | https://www.jstage.jst.go.jp/article/jhtb/22/3/22_293/_article | |||||
| 関連名称 | J-STAGE | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | https://doi.org/10.2485/jhtb.22.293 | |||||
| 関連名称 | info:doi/10.2485/jhtb.22.293 | |||||
| 医中誌ID | ||||||
| 識別子タイプ | ICHUSHI | |||||
| 関連識別子 | 2014231427 | |||||
| フォーマット | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | application/pdf | |||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
| 学位授与年度 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 2013 | |||||
| 報告番号 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 乙第27号 | |||||
| 学位授与番号 | ||||||
| 学位授与番号 | 乙第27号 | |||||
| 学位授与年月日 | ||||||
| 学位授与年月日 | 2014-03-06 | |||||
| 学位名 | ||||||
| 学位名 | 博士（歯学） | |||||
| 学位授与大学 | ||||||
| 学位授与機関識別子Scheme | kakenhi | |||||
| 学位授与機関識別子 | 33602 | |||||
| 学位授与機関名 | 松本歯科大学 | |||||
