{"_buckets": {"deposit": "c23bc8bb-d6fa-4510-a6b8-503354c425e4"}, "_deposit": {"created_by": 3, "id": "2282", "owners": [3], "pid": {"revision_id": 0, "type": "depid", "value": "2282"}, "status": "published"}, "_oai": {"id": "oai:mdu.repo.nii.ac.jp:00002282", "sets": ["187", "195", "270", "271", "272", "273", "274"]}, "author_link": ["2384", "2386", "2385", "2389", "2387", "2388"], "item_10001_biblio_info_7": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2012", "bibliographicIssueDateType": "Issued"}, "bibliographicPageStart": "11", "bibliographicVolumeNumber": "10", "bibliographic_titles": [{}, {"bibliographic_title": "Journal of Negative Results in BioMedicine", "bibliographic_titleLang": "en"}]}]}, "item_10001_description_19": {"attribute_name": "フォーマット", "attribute_value_mlt": [{"subitem_description": "application/pdf", "subitem_description_type": "Other"}]}, "item_10001_description_5": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "Objective: Periodontal disease is accompanied by inflammation of the gingiva and destruction of periodontal\ntissues, leading to alveolar bone loss in severe clinical cases. Interleukin (IL)-6, IL-8, and the chemical mediator\nprostaglandin E2 (PGE2) are known to play important roles in inflammatory responses and tissue degradation.\nRecently, we reported that the protein kinase A (PKA) inhibitor H-89 suppresses lipopolysaccharide (LPS)-induced\nIL-8 production by human gingival fibroblasts (HGFs). In the present study, the relevance of the PKA activity and\ntwo PKA-activating drugs, aminophylline and adrenaline, to LPS-induced inflammatory cytokines (IL-6 and IL-8) and\nPGE2 by HGFs were examined.\nMethods: HGFs were treated with LPS from Porphyromonas gingivalis and H-89, the cAMP analog dibutyryl cyclic\nAMP (dbcAMP), aminophylline, or adrenaline. After 24 h, IL-6, IL-8, and PGE2 levels were evaluated by ELISA.\nResults: H-89 did not affect LPS-induced IL-6 production, but suppressed IL-8 and PGE2 production. In contrast,\ndbcAMP significantly increased LPS-induced IL-6, IL-8, and PGE2 production. Up to 10 μg/ml of aminophylline did\nnot affect LPS-induced IL-6, IL-8, or PGE2 production, but they were significantly increased at 100 μg/ml. Similarly,\n0.01 μg/ml of adrenaline did not affect LPS-induced IL-6, IL-8, or PGE2 production, but they were significantly\nincreased at concentrations of 0.1 and 1 μg/ml. In the absence of LPS, H-89, dbcAMP, aminophylline, and\nadrenaline had no relevance to IL-6, IL-8, or PGE2 production.\nConclusion: These results suggest that the PKA pathway, and also PKA-activating drugs, enhance LPS-induced IL-6,\nIL-8, and PGE2 production by HGFs. However, aminophylline may not have an effect on the production of these\nmolecules at concentrations used in clinical settings (8 to 20 μg/ml in serum). These results suggest that\naminophylline does not affect inflammatory responses in periodontal disease.", "subitem_description_type": "Abstract"}]}, "item_10001_publisher_8": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "BioMed Central"}]}, "item_10001_relation_13": {"attribute_name": "PubMed番号", "attribute_value_mlt": [{"subitem_relation_type": "isIdenticalTo", "subitem_relation_type_id": {"subitem_relation_type_id_text": "info:pmid/22452847", "subitem_relation_type_select": "PMID"}}]}, "item_10001_relation_14": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_name": [{"subitem_relation_name_text": "info:doi/10.1186/1477-5751-11-10."}], "subitem_relation_type": "isIdenticalTo", "subitem_relation_type_id": {"subitem_relation_type_id_text": "https://doi.org/10.1186/1477-5751-11-10.", "subitem_relation_type_select": "DOI"}}]}, "item_10001_relation_16": {"attribute_name": "情報源", "attribute_value_mlt": [{"subitem_relation_name": [{"subitem_relation_name_text": "雑誌掲載論文(電子版)"}]}]}, "item_10001_relation_17": {"attribute_name": "関連サイト", "attribute_value_mlt": [{"subitem_relation_name": [{"subitem_relation_name_text": "BioMed Central Ltd"}], "subitem_relation_type_id": {"subitem_relation_type_id_text": "http://www.jnrbm.com/content/11/1/10", "subitem_relation_type_select": "URI"}}]}, "item_10001_rights_15": {"attribute_name": "権利", "attribute_value_mlt": [{"subitem_rights": "© 2012 Ara et al; licensee BioMed Central Ltd. "}]}, "item_10001_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "1477-5751", "subitem_source_identifier_type": "ISSN"}]}, "item_10001_version_type_20": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_970fb48d4fbd8a85", "subitem_version_type": "VoR"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "ARA, TOSHIAKI"}], "nameIdentifiers": [{"nameIdentifier": "2384", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "FUJINAMI, YOSHIAKI"}], "nameIdentifiers": [{"nameIdentifier": "2385", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "URANO, HIROKO"}], "nameIdentifiers": [{"nameIdentifier": "2386", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "HIRAI, KANAME"}], "nameIdentifiers": [{"nameIdentifier": "2387", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "HATTORI, TOSHIMI"}], "nameIdentifiers": [{"nameIdentifier": "2388", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "MIYAZAWA, HIROO"}], "nameIdentifiers": [{"nameIdentifier": "2389", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2014-10-06"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "2014-002_JA.pdf", "filesize": [{"value": "1.6 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_11", "mimetype": "application/pdf", "size": 1600000.0, "url": {"label": "2014-002_JA.pdf", "url": "https://mdu.repo.nii.ac.jp/record/2282/files/2014-002_JA.pdf"}, "version_id": "749d9782-e529-45a4-9cc7-d930a268c744"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "Protein kinase A", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "interleukin,", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "prostaglandin E2", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "human gingival fibroblast", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Cyclic AMP-Dependent Protein Kinases", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Dinoprostone", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Fibroblasts", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Gingiva", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Interleukin-6", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Interleukin-8", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Lipopolysaccharides", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Protein kinase A enhances lipopolysaccharideinduced IL-6, IL-8, and PGE2 production by human gingival broblasts", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Protein kinase A enhances lipopolysaccharideinduced IL-6, IL-8, and PGE2 production by human gingival broblasts", "subitem_title_language": "en"}]}, "item_type_id": "10001", "owner": "3", "path": ["187", "195", "270", "271", "272", "273", "274"], "permalink_uri": "https://mdu.repo.nii.ac.jp/records/2282", "pubdate": {"attribute_name": "公開日", "attribute_value": "2014-10-06"}, "publish_date": "2014-10-06", "publish_status": "0", "recid": "2282", "relation": {}, "relation_version_is_last": true, "title": ["Protein kinase A enhances lipopolysaccharideinduced IL-6, IL-8, and PGE2 production by human gingival broblasts"], "weko_shared_id": 3}